Possibility of Coagulation System Activation Determination with Tissue Factor in Pregnancy Complications
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F16%3A33159832" target="_blank" >RIV/61989592:15110/16:33159832 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61989592:15120/16:33159832
Výsledek na webu
<a href="http://dx.doi.org/10.7754/Clin.Lab.2016.151234" target="_blank" >http://dx.doi.org/10.7754/Clin.Lab.2016.151234</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7754/Clin.Lab.2016.151234" target="_blank" >10.7754/Clin.Lab.2016.151234</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Possibility of Coagulation System Activation Determination with Tissue Factor in Pregnancy Complications
Popis výsledku v původním jazyce
Background: In this part of the study, where we determined the causes of preeclampsia and other obstetric complications, we focused on the role of tissue factor (TF) in the activation of these pathophysiological processes. Recent findings attribute a significant part of the activation of coagulation creation of autoantibodies. Once this mechanism is activated, the antibodies induce expression of tissue factor (TF, CD142) on monocytes and vascular endothelial cells. Methods: We have proposed a monitor activation model of the coagulation system in preeclampsia and other pregnancy complications using TF expression on monocytes by flow cytometry and simultaneous determination the TF-induced thrombin generation in plasma. To determine expression of tissue factor (CD142) on monocytes, we proposed a method of multicolor flow cytometry using anti CD45 PerCP, anti CD14 APC, anti CD16b FITC, and anti CD142 PE antibodies and the corresponding isotype controls. Results: We verified the model on patients with severe antiphospholipid syndrome, which is a high expression of antibodies, in particular against beta-2GPI. Conclusions: We demonstrated complete inhibition of TF expression on monocytes and a significant reduction of thrombin generation in plasma.
Název v anglickém jazyce
Possibility of Coagulation System Activation Determination with Tissue Factor in Pregnancy Complications
Popis výsledku anglicky
Background: In this part of the study, where we determined the causes of preeclampsia and other obstetric complications, we focused on the role of tissue factor (TF) in the activation of these pathophysiological processes. Recent findings attribute a significant part of the activation of coagulation creation of autoantibodies. Once this mechanism is activated, the antibodies induce expression of tissue factor (TF, CD142) on monocytes and vascular endothelial cells. Methods: We have proposed a monitor activation model of the coagulation system in preeclampsia and other pregnancy complications using TF expression on monocytes by flow cytometry and simultaneous determination the TF-induced thrombin generation in plasma. To determine expression of tissue factor (CD142) on monocytes, we proposed a method of multicolor flow cytometry using anti CD45 PerCP, anti CD14 APC, anti CD16b FITC, and anti CD142 PE antibodies and the corresponding isotype controls. Results: We verified the model on patients with severe antiphospholipid syndrome, which is a high expression of antibodies, in particular against beta-2GPI. Conclusions: We demonstrated complete inhibition of TF expression on monocytes and a significant reduction of thrombin generation in plasma.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FD - Onkologie a hematologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/NT14394" target="_blank" >NT14394: Vliv exprese tkáňového faktoru na vznik těhotenských komplikací</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Clinical Laboratory
ISSN
1433-6510
e-ISSN
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Svazek periodika
62
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
6
Strana od-do
1851-1856
Kód UT WoS článku
000385336100003
EID výsledku v databázi Scopus
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