Enantioseparation of D,L-2-hydroxyglutaric acid by capillary electrophoresis with tandem mass spectrometry-Fast and efficient tool for D- and L-2-hydroxyglutaracidurias diagnosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F16%3A33161400" target="_blank" >RIV/61989592:15110/16:33161400 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/16:33161400

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0021967316307191" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0021967316307191</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2016.05.095" target="_blank" >10.1016/j.chroma.2016.05.095</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enantioseparation of D,L-2-hydroxyglutaric acid by capillary electrophoresis with tandem mass spectrometry-Fast and efficient tool for D- and L-2-hydroxyglutaracidurias diagnosis

Popis výsledku v původním jazyce

A novel capillary electrophoresis tandem mass spectrometry method for the enantioseparation and identification of 2-hydroxyglutaric acid enantiomers without derivatization for clinical purposes was described. Vancomycin chloride was used as an efficient chiral selector for the discrimination of 2-hydroxyglutaric acid enantiomers by capillary electrophoresis employed complete capillary filling method. The obtained resolution was 2.05. Hyphenation of CE with tandem mass spectrometry allows a reliable identification of separated enantiomers as well as their quantification. The method was validated and applied for the separation, identification and determination of 2-hydroxyglutaric enantiomers in urine samples obtained from healthy patients and two urine samples obtained from child patients suffering from high urine excretion of 2-hydroxyglutaric acid. Abnormal excretion of D-hydroxyglutaric acid was found in both child urine samples (104.5 +/- 2.1 and 2200.0 +/- 12.6 mmol/mol of creatinine, respectively). The limits of detection for D- and L-hydroxyglutaric acid were 31 and 38 nmol/L, respectively.

Název v anglickém jazyce

Enantioseparation of D,L-2-hydroxyglutaric acid by capillary electrophoresis with tandem mass spectrometry-Fast and efficient tool for D- and L-2-hydroxyglutaracidurias diagnosis

Popis výsledku anglicky

A novel capillary electrophoresis tandem mass spectrometry method for the enantioseparation and identification of 2-hydroxyglutaric acid enantiomers without derivatization for clinical purposes was described. Vancomycin chloride was used as an efficient chiral selector for the discrimination of 2-hydroxyglutaric acid enantiomers by capillary electrophoresis employed complete capillary filling method. The obtained resolution was 2.05. Hyphenation of CE with tandem mass spectrometry allows a reliable identification of separated enantiomers as well as their quantification. The method was validated and applied for the separation, identification and determination of 2-hydroxyglutaric enantiomers in urine samples obtained from healthy patients and two urine samples obtained from child patients suffering from high urine excretion of 2-hydroxyglutaric acid. Abnormal excretion of D-hydroxyglutaric acid was found in both child urine samples (104.5 +/- 2.1 and 2200.0 +/- 12.6 mmol/mol of creatinine, respectively). The limits of detection for D- and L-hydroxyglutaric acid were 31 and 38 nmol/L, respectively.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A: Symposium Volumes

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1467

Číslo periodika v rámci svazku

OCT

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

383-390

Kód UT WoS článku

000385323800036

EID výsledku v databázi Scopus

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