Metabolic Response of Visceral White Adipose Tissue of Obese Mice Exposed for 5 Days to Human Room Temperature Compared to Mouse Thermoneutrality

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F17%3A73580147" target="_blank" >RIV/61989592:15110/17:73580147 - isvavai.cz</a>

Výsledek na webu

<a href="https://obd.upol.cz/id_publ/333160007" target="_blank" >https://obd.upol.cz/id_publ/333160007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphys.2017.00179" target="_blank" >10.3389/fphys.2017.00179</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Metabolic Response of Visceral White Adipose Tissue of Obese Mice Exposed for 5 Days to Human Room Temperature Compared to Mouse Thermoneutrality

Popis výsledku v původním jazyce

Housing of laboratory mice at room temperature (RT) might be considered a constant cold stress, which induces a thermogenic program in brown adipose tissue. However, the early adaptive response of white adipose tissue, the fat storage organ of the body, to a change from thermoneutrality to RT is not known. This was investigated here for various WAT depots, focusing on epididymal WAT. Male adult diet-induced obese C57BL/6JOlaHsd mice housed at thermoneutrality, were for five days either switched to RT or remained at thermoneutrality. Energy metabolism was continuously measured using indirect calorimetry. At the end of the study, serum metabolomics and WAT transcriptomics were performed. We confirmed activation of the thermogenic program in 22ºC housed mice. Body weight and total fat mass were reduced. Whole body energy expenditure was increased, with a higher fatty acid to carbohydrate oxidation ratio and increased serum acylcarnitine levels, while energy intake was not significantly different between the two groups. Transcriptome analysis of eWAT identified tissue remodelling and inflammation as the most affected processes. Expression of pro-inflammatory M1 macrophage-related genes, and M1 over M2 macrophage ratio were decreased, which might be linked to an increased insulin sensitivity. Markers of thermogenesis were not altered in eWAT. Decreased expression of tryptophan hydroxylase 2 and cholecystokinin might represent altered neuroendocrine signalling. eWAT itself does not show increased fatty acid oxidation. The three measured WATs, epididymal, mesenteric, and retroperitoneal, showed mainly similar responses; reduced inflammation, decreased carbohydrate oxidation, and no or small differences in fatty acid oxidation. However, Ucp1 was only expressed and increased in rWAT in 22ᵒC housed mice. Cck expression was decreased in the three WATs, significantly in eWAT and rWAT, in contrast to Tph2, which was decreased in eWAT while not expressed in mWAT and rWAT.

Název v anglickém jazyce

Metabolic Response of Visceral White Adipose Tissue of Obese Mice Exposed for 5 Days to Human Room Temperature Compared to Mouse Thermoneutrality

Popis výsledku anglicky

Housing of laboratory mice at room temperature (RT) might be considered a constant cold stress, which induces a thermogenic program in brown adipose tissue. However, the early adaptive response of white adipose tissue, the fat storage organ of the body, to a change from thermoneutrality to RT is not known. This was investigated here for various WAT depots, focusing on epididymal WAT. Male adult diet-induced obese C57BL/6JOlaHsd mice housed at thermoneutrality, were for five days either switched to RT or remained at thermoneutrality. Energy metabolism was continuously measured using indirect calorimetry. At the end of the study, serum metabolomics and WAT transcriptomics were performed. We confirmed activation of the thermogenic program in 22ºC housed mice. Body weight and total fat mass were reduced. Whole body energy expenditure was increased, with a higher fatty acid to carbohydrate oxidation ratio and increased serum acylcarnitine levels, while energy intake was not significantly different between the two groups. Transcriptome analysis of eWAT identified tissue remodelling and inflammation as the most affected processes. Expression of pro-inflammatory M1 macrophage-related genes, and M1 over M2 macrophage ratio were decreased, which might be linked to an increased insulin sensitivity. Markers of thermogenesis were not altered in eWAT. Decreased expression of tryptophan hydroxylase 2 and cholecystokinin might represent altered neuroendocrine signalling. eWAT itself does not show increased fatty acid oxidation. The three measured WATs, epididymal, mesenteric, and retroperitoneal, showed mainly similar responses; reduced inflammation, decreased carbohydrate oxidation, and no or small differences in fatty acid oxidation. However, Ucp1 was only expressed and increased in rWAT in 22ᵒC housed mice. Cck expression was decreased in the three WATs, significantly in eWAT and rWAT, in contrast to Tph2, which was decreased in eWAT while not expressed in mWAT and rWAT.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/LO1304" target="_blank" >LO1304: Podpora udržitelnosti Ústavu molekulární a translační medicíny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Physiology

ISSN

1664-042X

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

179 (23 March 2017)

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

1-15

Kód UT WoS článku

000397087800002

EID výsledku v databázi Scopus

—