Infiltration of Prostate Cancer by CD204 and CD3 Cells Correlates with ERG Expression and TMPRSS2-ERG Gene Fusion

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F18%3A73591088" target="_blank" >RIV/61989592:15110/18:73591088 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.14735/amko2018421" target="_blank" >http://dx.doi.org/10.14735/amko2018421</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14735/amko2018421" target="_blank" >10.14735/amko2018421</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Infiltration of Prostate Cancer by CD204 and CD3 Cells Correlates with ERG Expression and TMPRSS2-ERG Gene Fusion

Popis výsledku v původním jazyce

The aim of the study was to detect CD204 + and CD3 + cells in the infiltrate of benign prostatic hyperplasia, prostatic intraepithelial neoplasia and prostatic cancer in prostate specimens after radical prostatectomy. Another goal was to determine correlation of the intensity of the infiltration with ERG oncoprotein expression as well as with the presence of activating translocation TMPRSS2-ERG. Materials and Methods: To confirm the translocation, we used fluorescence in situ hybridization. Imunohistochemistry was used to detect the presence of ERG oncoprotein and for assesment of the number of CD204+ and CD3+ infiltrating cells. We determined the capability to infiltrate malignant structures according to differences in infiltration of benign and malignant prostate structures. Results: Biometric analysis confirmed that the number of CD204+ macrophages in the malignant structure was significantly higher than in the benign prostatic hyperplasia regardless of the fusion pattern. Increased infiltration by CD3+ cells was only detected in malignant structures of the prostate in a group with normal signal pattern and in a group with TMPRSS2-ERG fusion. Expression of ERG positively correlated with CD204+ and CD3+ cells infiltration of malignant structures only in cases where the TMPRSS2-ERG fusion was found.

Název v anglickém jazyce

Infiltration of Prostate Cancer by CD204 and CD3 Cells Correlates with ERG Expression and TMPRSS2-ERG Gene Fusion

Popis výsledku anglicky

The aim of the study was to detect CD204 + and CD3 + cells in the infiltrate of benign prostatic hyperplasia, prostatic intraepithelial neoplasia and prostatic cancer in prostate specimens after radical prostatectomy. Another goal was to determine correlation of the intensity of the infiltration with ERG oncoprotein expression as well as with the presence of activating translocation TMPRSS2-ERG. Materials and Methods: To confirm the translocation, we used fluorescence in situ hybridization. Imunohistochemistry was used to detect the presence of ERG oncoprotein and for assesment of the number of CD204+ and CD3+ infiltrating cells. We determined the capability to infiltrate malignant structures according to differences in infiltration of benign and malignant prostate structures. Results: Biometric analysis confirmed that the number of CD204+ macrophages in the malignant structure was significantly higher than in the benign prostatic hyperplasia regardless of the fusion pattern. Increased infiltration by CD3+ cells was only detected in malignant structures of the prostate in a group with normal signal pattern and in a group with TMPRSS2-ERG fusion. Expression of ERG positively correlated with CD204+ and CD3+ cells infiltration of malignant structures only in cases where the TMPRSS2-ERG fusion was found.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

30217 - Urology and nephrology

Projekt

<a href="/cs/project/LO1304" target="_blank" >LO1304: Podpora udržitelnosti Ústavu molekulární a translační medicíny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Klinicka Onkologie

ISSN

0862-495X

e-ISSN

—

Svazek periodika

31

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

421-428

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85058751990