Nanodiamonds of different sizes and charges show different ability to stimulate and mature murine dendritic cells in vitro

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nanodiamonds of different sizes and charges show different ability to stimulate and mature murine dendritic cells in vitro

Popis výsledku v původním jazyce

Nanodiamonds represent nanoparticles with various properties depending on their size, charge, shape, surface modification atc. They were modified to exhibit very stable fluorescence that could help by tracking of desired molecules coupled to the nanodiamonds. Nanodiamonds of different sizes with different charges were tested in this analysis. Particles ranging from 5 to 80 nm with a positive or negative surface charge were examined for their immunostimulatory properties. Murine bone marrow-derived dendriticcells were pulsed for 24 hours with these construct and different maturation marker were determined by flow cytometry including CD11c, CD80, CD86, CD40 as well as MHCI and MHCII molecules. Our data show that all the types of nanodiamonds stimulate BMDCs very slightly in comparison to lipopolysaccharide (LPS). Only moderate increase in CD86 and CD80 maturation markers was observed. On the other hand it is interesting that all the sizes of diamonds stimulated expression of MHCI molecules comparably to LPS and limited expression of MHCII molecules when compared to LPS or not pulsed control. After analyzing the effect of nanodiamonds surface charge on pulsed BMDC response, generally the positive charge is more effective in BMDCs stimulation (CD86 and CD80). In contrast the negative charge increases significantly the MHCI expression of dendritic cells. Finally, all types of nanodiamonds-or LPS-stimulated BMDCs and control cells (not pulsed BMDCs) were analyzed using the light microscopy. The nanodiamonds of sizes between 40 and 80 nm seemed to be toxicfor the BMDCs, as those exposed to nanodiamonds have had almostno dendrites and many of them have been destroyed especially when the 80 nm size of nanodiamond was used. Here both immunomodulatory and toxic effects of nanodiamonds were reported dependent on the nature of analyzed nanodiamonds. This needs to be considered in opting their application in biologic systems including humans.

Název v anglickém jazyce

Nanodiamonds of different sizes and charges show different ability to stimulate and mature murine dendritic cells in vitro

Popis výsledku anglicky

Nanodiamonds represent nanoparticles with various properties depending on their size, charge, shape, surface modification atc. They were modified to exhibit very stable fluorescence that could help by tracking of desired molecules coupled to the nanodiamonds. Nanodiamonds of different sizes with different charges were tested in this analysis. Particles ranging from 5 to 80 nm with a positive or negative surface charge were examined for their immunostimulatory properties. Murine bone marrow-derived dendriticcells were pulsed for 24 hours with these construct and different maturation marker were determined by flow cytometry including CD11c, CD80, CD86, CD40 as well as MHCI and MHCII molecules. Our data show that all the types of nanodiamonds stimulate BMDCs very slightly in comparison to lipopolysaccharide (LPS). Only moderate increase in CD86 and CD80 maturation markers was observed. On the other hand it is interesting that all the sizes of diamonds stimulated expression of MHCI molecules comparably to LPS and limited expression of MHCII molecules when compared to LPS or not pulsed control. After analyzing the effect of nanodiamonds surface charge on pulsed BMDC response, generally the positive charge is more effective in BMDCs stimulation (CD86 and CD80). In contrast the negative charge increases significantly the MHCI expression of dendritic cells. Finally, all types of nanodiamonds-or LPS-stimulated BMDCs and control cells (not pulsed BMDCs) were analyzed using the light microscopy. The nanodiamonds of sizes between 40 and 80 nm seemed to be toxicfor the BMDCs, as those exposed to nanodiamonds have had almostno dendrites and many of them have been destroyed especially when the 80 nm size of nanodiamond was used. Here both immunomodulatory and toxic effects of nanodiamonds were reported dependent on the nature of analyzed nanodiamonds. This needs to be considered in opting their application in biologic systems including humans.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

30102 - Immunology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

11th International Conference Drug Delivery Systems Nanotechnology for Healthcare: Progress in Recombinant Vaccines, Molecular Adjuvants, Modern Drug Delivery Systems and Cell Therapy

ISBN

978-80-907074-6-7

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

12

Strana od-do

59-70

Název nakladatele

nakladatelství Machovský

Místo vydání

Olomouc

Místo konání akce

Telč

Datum konání akce

5. 6. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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