Study of chromium species release from metal implants in blood and joint effusion: Utilization of HPLC-ICP-MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F18%3A73591359" target="_blank" >RIV/61989592:15110/18:73591359 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/18:73591359

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0039914018303424" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914018303424</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2018.03.100" target="_blank" >10.1016/j.talanta.2018.03.100</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Study of chromium species release from metal implants in blood and joint effusion: Utilization of HPLC-ICP-MS

Popis výsledku v původním jazyce

The objective of this study was to develop and validate a novel analytical procedure for determination of total chromium and Cr(III) and Cr(VI) species released from metal implants into whole blood and joint effusion. Firstly, the ion-pair chromatographic method employing reversed-phase high-performance liquid chromatography inductively coupled plasma mass spectrometry (ICP-MS) for analysis of species was developed. Secondly, all samples, protein and low molecular fractions were analyzed for their total chromium content using ICP-MS. This new measurement procedure was validated by the following parameters: limit of detection (0.13 mu g L-1 for Cr(III), 0.14 mu g L-1 for Cr(VI)), linearity of calibration, trueness (recovery 84-92%), intermediate precision (RSD &lt; 5%). We determined statistically significantly higher chromium levels in joint effusion samples obtained from patients in comparison with a control group. On the other hand, no relevant difference among the concentrations of both species and total chromium in blood was observed. Our results show that the majority of chromium is present in the trivalent form and bound to proteins. This speciation study is rare in the field of speciation analysis in clinical samples. It is characterized by very fast and simple sample preparation without any changes in distribution or stability of both Cr forms and efficient simultaneous analysis of Cr species.

Název v anglickém jazyce

Study of chromium species release from metal implants in blood and joint effusion: Utilization of HPLC-ICP-MS

Popis výsledku anglicky

The objective of this study was to develop and validate a novel analytical procedure for determination of total chromium and Cr(III) and Cr(VI) species released from metal implants into whole blood and joint effusion. Firstly, the ion-pair chromatographic method employing reversed-phase high-performance liquid chromatography inductively coupled plasma mass spectrometry (ICP-MS) for analysis of species was developed. Secondly, all samples, protein and low molecular fractions were analyzed for their total chromium content using ICP-MS. This new measurement procedure was validated by the following parameters: limit of detection (0.13 mu g L-1 for Cr(III), 0.14 mu g L-1 for Cr(VI)), linearity of calibration, trueness (recovery 84-92%), intermediate precision (RSD &lt; 5%). We determined statistically significantly higher chromium levels in joint effusion samples obtained from patients in comparison with a control group. On the other hand, no relevant difference among the concentrations of both species and total chromium in blood was observed. Our results show that the majority of chromium is present in the trivalent form and bound to proteins. This speciation study is rare in the field of speciation analysis in clinical samples. It is characterized by very fast and simple sample preparation without any changes in distribution or stability of both Cr forms and efficient simultaneous analysis of Cr species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TALANTA

ISSN

0039-9140

e-ISSN

—

Svazek periodika

185

Číslo periodika v rámci svazku

AUG

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

370-377

Kód UT WoS článku

000433656200050

EID výsledku v databázi Scopus

2-s2.0-85044963646