Human keratinocyte cell line as a suitable alternative model for in vitro phototoxicity testing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73594099" target="_blank" >RIV/61989592:15110/19:73594099 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962019000100021&lng=en&nrm=iso&tlng=en" target="_blank" >http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962019000100021&lng=en&nrm=iso&tlng=en</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1590/abd1806-4841.20197620" target="_blank" >10.1590/abd1806-4841.20197620</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Human keratinocyte cell line as a suitable alternative model for in vitro phototoxicity testing

Popis výsledku v původním jazyce

The 3T3 Neutral Red Uptake Phototoxicity Test (3T3NRUPT) is the only validated in vitro assay for compound phototoxicity testing that replaces the use of animals for testing cosmetics, which is banned in Europe. 3T3NRUPT uses the mouse fibroblast cell line Balb/c 3T3 (Balb/c). When the test was introduced in our studies, we dealt with a problem with Balb/c detachment from culture plates during the washing steps and following incubation in phosphate buffered saline (PBS). In order to improve the model system, we compared the attachment of Balb/c on various plate surfaces. We also tested the effect of different buffered saline solutions. In parallel, experiments were performed on a human keratinocyte cell line (HaCaT). The response of both cell lines to chlorpromazine, a recommended phototoxic compound in the validated 3T3NRUPT protocol, was also compared. Results showed a dramatic reduction in the number of Balb/c after the washing steps and subsequent incubation in buffered saline solution. In contrast, HaCaT cells were minimally affected. No beneficial effect on Balb/c number was found for any of the tested surfaces and/or buffered saline solutions. In conclusion, HaCaT cells are minimally affected during the phototoxicity assay and seems to be a suitable alternative alongside Balb/c especially for testing compounds intended to be applied topically.

Název v anglickém jazyce

Human keratinocyte cell line as a suitable alternative model for in vitro phototoxicity testing

Popis výsledku anglicky

The 3T3 Neutral Red Uptake Phototoxicity Test (3T3NRUPT) is the only validated in vitro assay for compound phototoxicity testing that replaces the use of animals for testing cosmetics, which is banned in Europe. 3T3NRUPT uses the mouse fibroblast cell line Balb/c 3T3 (Balb/c). When the test was introduced in our studies, we dealt with a problem with Balb/c detachment from culture plates during the washing steps and following incubation in phosphate buffered saline (PBS). In order to improve the model system, we compared the attachment of Balb/c on various plate surfaces. We also tested the effect of different buffered saline solutions. In parallel, experiments were performed on a human keratinocyte cell line (HaCaT). The response of both cell lines to chlorpromazine, a recommended phototoxic compound in the validated 3T3NRUPT protocol, was also compared. Results showed a dramatic reduction in the number of Balb/c after the washing steps and subsequent incubation in buffered saline solution. In contrast, HaCaT cells were minimally affected. No beneficial effect on Balb/c number was found for any of the tested surfaces and/or buffered saline solutions. In conclusion, HaCaT cells are minimally affected during the phototoxicity assay and seems to be a suitable alternative alongside Balb/c especially for testing compounds intended to be applied topically.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA15-10897S" target="_blank" >GA15-10897S: Vliv obsahových složek silymarinu na účinky UVA záření vyvolané v kožních buňkách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ANAIS BRASILEIROS DE DERMATOLOGIA

ISSN

0365-0596

e-ISSN

—

Svazek periodika

94

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

BR - Brazilská federativní republika

Počet stran výsledku

2

Strana od-do

105-106

Kód UT WoS článku

000457571600021

EID výsledku v databázi Scopus

2-s2.0-85061159042