A method of determining the amount of DNA in sample

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73594376" target="_blank" >RIV/61989592:15110/19:73594376 - isvavai.cz</a>

Výsledek na webu

<a href="https://worldwide.espacenet.com/patent/search/family/062791482/publication/EP3395956A1?q=EP%203395956" target="_blank" >https://worldwide.espacenet.com/patent/search/family/062791482/publication/EP3395956A1?q=EP%203395956</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A method of determining the amount of DNA in sample

Popis výsledku v původním jazyce

The invention concerns a method of determining the amount of DNA in samples, comprising the following steps: (i) a sample is incubated in a solution of at least one fluorescent substance selected from the group consisted of DAPI, Hoechst 33258, Hoechst 33342 or Hoechst 34580, which, in the course of this incubation, binds to the DNA present in the sample; (ii) the sample is washed with a washing solution and/or water, which washed away the fluorescent substances not bound to the DNA in the sample; (iii) the fluorescent substances bound to the DNA in the sample are eluted from the DNA with an aqueous elution solution of at least one substance selected from the group consisted of SDS, LiDS and sarcosyl, wherein the concentration of SDS, LiDS and sarcosyl is at least 0.1 % (w/v); (iv) the amount of DNA is determined in the elution solution from the previous step by measuring the fluorescent signal of the fluorescent substance used in step (i). The invention further relates to a method of determining the amount of EdU and/or BrdU and/or CldU and/or IdU incorporated into DNA and/or the content of proteins and/or the amount of RNA and/or the amount of specific DNA sequences and/or the amount of sugars and/or the amount of lipids and/or the amount of fluorescently-labelled DNA and/or fluorescently-labelled RNA and/or fluorescently-labelled proteins in cells and tissues; to a method of determining the replication activity in cells and tissues.

Název v anglickém jazyce

A method of determining the amount of DNA in sample

Popis výsledku anglicky

The invention concerns a method of determining the amount of DNA in samples, comprising the following steps: (i) a sample is incubated in a solution of at least one fluorescent substance selected from the group consisted of DAPI, Hoechst 33258, Hoechst 33342 or Hoechst 34580, which, in the course of this incubation, binds to the DNA present in the sample; (ii) the sample is washed with a washing solution and/or water, which washed away the fluorescent substances not bound to the DNA in the sample; (iii) the fluorescent substances bound to the DNA in the sample are eluted from the DNA with an aqueous elution solution of at least one substance selected from the group consisted of SDS, LiDS and sarcosyl, wherein the concentration of SDS, LiDS and sarcosyl is at least 0.1 % (w/v); (iv) the amount of DNA is determined in the elution solution from the previous step by measuring the fluorescent signal of the fluorescent substance used in step (i). The invention further relates to a method of determining the amount of EdU and/or BrdU and/or CldU and/or IdU incorporated into DNA and/or the content of proteins and/or the amount of RNA and/or the amount of specific DNA sequences and/or the amount of sugars and/or the amount of lipids and/or the amount of fluorescently-labelled DNA and/or fluorescently-labelled RNA and/or fluorescently-labelled proteins in cells and tissues; to a method of determining the replication activity in cells and tissues.

Druh

P - Patent

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.

Číslo patentu nebo vzoru

3395956

Vydavatel

EPO_1 -

Název vydavatele

European Patent Office

Místo vydání

Munich, The Hague, Berlin, Vienna, Brussels

Stát vydání

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Datum přijetí

13. 11. 2019

Název vlastníka

Univerzita Palackého v Olomouci

Způsob využití

A - Výsledek využívá pouze poskytovatel

Druh možnosti využití

A - K využití výsledku jiným subjektem je vždy nutné nabytí licence