N-acetylcysteine dual and antagonistic effect on cadmium cytotoxicity in human leukemia cells.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73597952" target="_blank" >RIV/61989592:15110/19:73597952 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S1382668919300845?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1382668919300845?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.etap.2019.103213." target="_blank" >10.1016/j.etap.2019.103213.</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
N-acetylcysteine dual and antagonistic effect on cadmium cytotoxicity in human leukemia cells.
Popis výsledku v původním jazyce
Although cadmium (Cd2+) is unable to form reactive oxygen species (ROS) directly, many of its adverse effects are connected to increased ROS generation resulting in cell death. In support of this supposition, a large number of studies have shown protective effects of antioxidants such as N-acetylcysteine (NAC) against cadmium induced cytotoxicity. Here, we describe the cytotoxic effects of Cd2+ on human leukemia U937 and K562 cells that were not mediated by oxidative stress. Surprisingly, we observed that addition of low concentrations of NAC can drastically potentiate cadmium cytotoxicity solely via ROS production. However, all adverse effects of the metal were prevented by NAC at high concentrations. Detailed analysis indicated that the protective effect of NAC was mediated by its ability to form stable complex with cadmium [Cd(NAC)2]. In conclusion, NAC exhibits dual and antagonistic effects on Cd2+ cytotoxicity in human leukemia cells.
Název v anglickém jazyce
N-acetylcysteine dual and antagonistic effect on cadmium cytotoxicity in human leukemia cells.
Popis výsledku anglicky
Although cadmium (Cd2+) is unable to form reactive oxygen species (ROS) directly, many of its adverse effects are connected to increased ROS generation resulting in cell death. In support of this supposition, a large number of studies have shown protective effects of antioxidants such as N-acetylcysteine (NAC) against cadmium induced cytotoxicity. Here, we describe the cytotoxic effects of Cd2+ on human leukemia U937 and K562 cells that were not mediated by oxidative stress. Surprisingly, we observed that addition of low concentrations of NAC can drastically potentiate cadmium cytotoxicity solely via ROS production. However, all adverse effects of the metal were prevented by NAC at high concentrations. Detailed analysis indicated that the protective effect of NAC was mediated by its ability to form stable complex with cadmium [Cd(NAC)2]. In conclusion, NAC exhibits dual and antagonistic effects on Cd2+ cytotoxicity in human leukemia cells.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
ISSN
1382-6689
e-ISSN
—
Svazek periodika
2019
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
1-9
Kód UT WoS článku
000487768200011
EID výsledku v databázi Scopus
2-s2.0-85068433916