BODIPY-aza-indole derivate complex as a selective fluorescent sensor for autolysosomes detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F22%3A73616491" target="_blank" >RIV/61989592:15110/22:73616491 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/22:73616491

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0925400521015094" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0925400521015094</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2021.130941" target="_blank" >10.1016/j.snb.2021.130941</a>

Jazyk výsledku

angličtina

Název v původním jazyce

BODIPY-aza-indole derivate complex as a selective fluorescent sensor for autolysosomes detection

Popis výsledku v původním jazyce

Autophagy is a process of removal and degradation of unneeded or damaged cellular components, which plays an essential role in several physiological processes and associated diseases or ageing. Despite its importance, a simply applicable selective fluorescent dye able to specifically stain autolysosomes as a particular component of autophagy does not exist. Addressing this challenge, we present a new BODIPY-aza-indole complex (BODIPYAZD-1) as a fluorescent dye selectively staining autolysosomes and distinguish them from lysosomes and autophagosomes. Taking advantage of BODIPY, this compound has excellent fluorescent properties, it is resistant to photobleaching, it is not phototoxic, and it is compatible with live-cell, time-lapse, and super-resolution microscopy and fixation. Due to the simple staining procedure and non-toxicity, we suggest this compound for studies of later phases of autophagy as well as for observation of autolysosome physiology in live and/or fixed cells.

Název v anglickém jazyce

BODIPY-aza-indole derivate complex as a selective fluorescent sensor for autolysosomes detection

Popis výsledku anglicky

Autophagy is a process of removal and degradation of unneeded or damaged cellular components, which plays an essential role in several physiological processes and associated diseases or ageing. Despite its importance, a simply applicable selective fluorescent dye able to specifically stain autolysosomes as a particular component of autophagy does not exist. Addressing this challenge, we present a new BODIPY-aza-indole complex (BODIPYAZD-1) as a fluorescent dye selectively staining autolysosomes and distinguish them from lysosomes and autophagosomes. Taking advantage of BODIPY, this compound has excellent fluorescent properties, it is resistant to photobleaching, it is not phototoxic, and it is compatible with live-cell, time-lapse, and super-resolution microscopy and fixation. Due to the simple staining procedure and non-toxicity, we suggest this compound for studies of later phases of autophagy as well as for observation of autolysosome physiology in live and/or fixed cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

SENSORS AND ACTUATORS B-CHEMICAL

ISSN

0925-4005

e-ISSN

—

Svazek periodika

351

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

6

Strana od-do

"130941-1"-"130941-6"

Kód UT WoS článku

000720837000006

EID výsledku v databázi Scopus

2-s2.0-85118475592