Challenges of in situ hybridization in miRNA analysis of triple-negative breast cancer morphological diversity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F23%3A73622909" target="_blank" >RIV/61989592:15110/23:73622909 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00098892:_____/23:10158328

Výsledek na webu

<a href="http://www.elis.sk/download_file.php?product_id=7984&session_id=g6874af8plegej0p8k17jsqs42" target="_blank" >http://www.elis.sk/download_file.php?product_id=7984&session_id=g6874af8plegej0p8k17jsqs42</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/neo_2023_220519N533" target="_blank" >10.4149/neo_2023_220519N533</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Challenges of in situ hybridization in miRNA analysis of triple-negative breast cancer morphological diversity

Popis výsledku v původním jazyce

miRNA expression in triple-negative breast cancers (TNBC) has mainly been studied from a methodological viewpoint.However, it has not been considered that miRNA expression profile may be associated with a specific morphological entityinside every tumor. The verification of this hypothesis on a set of 25 TNBCs was the subject of our previous work, wherewe confirmed specific expression of the studied miRNAs in 82 samples of different morphologies including inflammatoryinfiltrate, spindle cell, clear cell, and metastases after RNA extraction and purification as well as microchip and biostatistical analysis. In the current work, we demonstrate a low suitability of in situ hybridization method for miRNA detectioncompared to RT-qPCR, and in detail discuss the biological role of 8 miRNAs with the most significant changes of expression.

Název v anglickém jazyce

Challenges of in situ hybridization in miRNA analysis of triple-negative breast cancer morphological diversity

Popis výsledku anglicky

miRNA expression in triple-negative breast cancers (TNBC) has mainly been studied from a methodological viewpoint.However, it has not been considered that miRNA expression profile may be associated with a specific morphological entityinside every tumor. The verification of this hypothesis on a set of 25 TNBCs was the subject of our previous work, wherewe confirmed specific expression of the studied miRNAs in 82 samples of different morphologies including inflammatoryinfiltrate, spindle cell, clear cell, and metastases after RNA extraction and purification as well as microchip and biostatistical analysis. In the current work, we demonstrate a low suitability of in situ hybridization method for miRNA detectioncompared to RT-qPCR, and in detail discuss the biological role of 8 miRNAs with the most significant changes of expression.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

<a href="/cs/project/NV16-31997A" target="_blank" >NV16-31997A: Diagnostické a prognostické dopady korelace miRNA expresního profilu s morfologickou heterogenitou u "triple-negative" karcinomů prsu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NEOPLASMA

ISSN

0028-2685

e-ISSN

1338-4317

Svazek periodika

70

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

9

Strana od-do

179-187

Kód UT WoS článku

001070933100001

EID výsledku v databázi Scopus

2-s2.0-85160206806