Isolation of mesophyll protoplasts of Cucumis spp. and Cucurbita spp.

Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F00%3A00001058" target="_blank" >RIV/61989592:15310/00:00001058 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Jazyk výsledku
angličtina
Název v původním jazyce
Isolation of mesophyll protoplasts of Cucumis spp. and Cucurbita spp.
Popis výsledku v původním jazyce
Well-developed first or second true leaves, originating from in vitro plants of two Cucumis species (C. melo, C. metuliferus) and three Cucurbita species (C. ficifolia, C. maxima, C. pepo), were ussed for protoplast isolation. The optimal composition ofthe enzyme solution and the most suitable isolation method were studied. The density and viability of protoplasts were used as efficiency criteria. the best isolation method and the enzyme solution were more suitable for Cucumis spp. than for Cucurbita spp., as the former had more viable and less damaged (plasmolyzed) protoplasts.
Název v anglickém jazyce
Isolation of mesophyll protoplasts of Cucumis spp. and Cucurbita spp.
Popis výsledku anglicky
Well-developed first or second true leaves, originating from in vitro plants of two Cucumis species (C. melo, C. metuliferus) and three Cucurbita species (C. ficifolia, C. maxima, C. pepo), were ussed for protoplast isolation. The optimal composition ofthe enzyme solution and the most suitable isolation method were studied. The density and viability of protoplasts were used as efficiency criteria. the best isolation method and the enzyme solution were more suitable for Cucumis spp. than for Cucurbita spp., as the former had more viable and less damaged (plasmolyzed) protoplasts.

Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EF - Botanika
OECD FORD obor
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Rok uplatnění
2000
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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