Endogeneous inhibitor of amine oxidase from the seedlings of Phaseolus vulgaris

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F00%3A00001073" target="_blank" >RIV/61989592:15310/00:00001073 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Endogeneous inhibitor of amine oxidase from the seedlings of Phaseolus vulgaris

Popis výsledku v původním jazyce

Copper amine oxidases (Cu-AO, amine:O2 oxidoreductase) is the key enzyme in the catabolism of biogenic amines. Cu-AO are inhibited by a variety of diverse natural or synthetic substances but any endogenous inhibitor of Cu-AO regulating its activity in vivo has been unambiguously characterized. In our previous studies, we confirmed Cu-AO from etiolated seedling of white bean (Phaseolus vulgaris) to be inhibited by a naturally occurring low-molecular endogenous inhibitor. To enable a detailed chemical andenzymological characterization of the inhibitor, we designed a bioassay-directed fractionation scheme of the seedling extract on the preparative scale. The purified substance was characterized as a reversible non-competitive inhibitor of Cu-AO from Phaseolus vulgaris, Pisum sativum and Lathyrus sativus.

Název v anglickém jazyce

Endogeneous inhibitor of amine oxidase from the seedlings of Phaseolus vulgaris

Popis výsledku anglicky

Copper amine oxidases (Cu-AO, amine:O2 oxidoreductase) is the key enzyme in the catabolism of biogenic amines. Cu-AO are inhibited by a variety of diverse natural or synthetic substances but any endogenous inhibitor of Cu-AO regulating its activity in vivo has been unambiguously characterized. In our previous studies, we confirmed Cu-AO from etiolated seedling of white bean (Phaseolus vulgaris) to be inhibited by a naturally occurring low-molecular endogenous inhibitor. To enable a detailed chemical andenzymological characterization of the inhibitor, we designed a bioassay-directed fractionation scheme of the seedling extract on the preparative scale. The purified substance was characterized as a reversible non-competitive inhibitor of Cu-AO from Phaseolus vulgaris, Pisum sativum and Lathyrus sativus.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemické listy

ISSN

0009-2770

e-ISSN

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Svazek periodika

94

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

XX - osoby bez státní příslušnosti

Počet stran výsledku

2

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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