A molecular survey to identify phytoplasmas associated with apple trees showing different disease symptoms.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F01%3A00001190" target="_blank" >RIV/61989592:15310/01:00001190 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A molecular survey to identify phytoplasmas associated with apple trees showing different disease symptoms.

Popis výsledku v původním jazyce

Apple trees showing a diverse range of symptoms from the Czech Republic and the UK were tested several times during the year to monitor and idntify phytoplasmas, The trees were examinated by nested PCR using broad spectrum or group specific primers to amplify phytoplasma 16SrDNA, or 16SrDNA plus spacer region. The identity of PCR products was confirmed by RFLP analyses. Several cutivars from the Czech Republic showing symptoms of rubbery wood, apple proliferation, or leaf deformation and reddening wereexamined, together with plants of the cv Lord Lambourne from the UK showing rubbery wood symptoms. Of the plants tested only a few were positive in nested PCR. The results suggest that appe trees can harbour phytoplasmas from group 16SrI-B, 16SrI-C, 16 SrX-a and 16SrXII-A. It is also apparent that phytoplasmas belonging to 16SrX-A group, typically associated with apple proliferation symptoms, could also occur in symptomless apple trees or in trees with symptoms other than typical apple pr

Název v anglickém jazyce

A molecular survey to identify phytoplasmas associated with apple trees showing different disease symptoms.

Popis výsledku anglicky

Apple trees showing a diverse range of symptoms from the Czech Republic and the UK were tested several times during the year to monitor and idntify phytoplasmas, The trees were examinated by nested PCR using broad spectrum or group specific primers to amplify phytoplasma 16SrDNA, or 16SrDNA plus spacer region. The identity of PCR products was confirmed by RFLP analyses. Several cutivars from the Czech Republic showing symptoms of rubbery wood, apple proliferation, or leaf deformation and reddening wereexamined, together with plants of the cv Lord Lambourne from the UK showing rubbery wood symptoms. Of the plants tested only a few were positive in nested PCR. The results suggest that appe trees can harbour phytoplasmas from group 16SrI-B, 16SrI-C, 16 SrX-a and 16SrXII-A. It is also apparent that phytoplasmas belonging to 16SrX-A group, typically associated with apple proliferation symptoms, could also occur in symptomless apple trees or in trees with symptoms other than typical apple pr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Svazek periodika

N

Číslo periodika v rámci svazku

550

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

371-376

Kód UT WoS článku

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EID výsledku v databázi Scopus

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