Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F01%3A00001214" target="_blank" >RIV/61989592:15310/01:00001214 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes.

Popis výsledku v původním jazyce

The dioecious white campion Silene latifolia (syn. Melandrium album) has heteromorphic sex chromosomes, XX in females and XY in males, that are larger than the autosomes and enable their separation by flow sorting. The group of MROS-genes, the first male-specifically expressed genes in dioecious plants, was recently identified in S. latifolia. To localize the MROSgenes, we used the flow-sorted X chromosomes and autosomes as a template for PCR with internal primers. Our results indicate that the MROS_Y gene is located in at least two copies tandemly arranged on the X chromosome with additional copy(ies) on the autosome(s), while MROS1, MROS2, and MROS4 are exclusively autosomal. The specificity of PCR products was checked by digestion with a restrictionenzyme or reamplification using nested primers. Homology search of databases has shown the presence of five MROS3 homologues in A. thaliana, four of them arranged in two tandems, each consisting of two copies. We conclude that MROS3 is a

Název v anglickém jazyce

Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes.

Popis výsledku anglicky

The dioecious white campion Silene latifolia (syn. Melandrium album) has heteromorphic sex chromosomes, XX in females and XY in males, that are larger than the autosomes and enable their separation by flow sorting. The group of MROS-genes, the first male-specifically expressed genes in dioecious plants, was recently identified in S. latifolia. To localize the MROSgenes, we used the flow-sorted X chromosomes and autosomes as a template for PCR with internal primers. Our results indicate that the MROS_Y gene is located in at least two copies tandemly arranged on the X chromosome with additional copy(ies) on the autosome(s), while MROS1, MROS2, and MROS4 are exclusively autosomal. The specificity of PCR products was checked by digestion with a restrictionenzyme or reamplification using nested primers. Homology search of databases has shown the presence of five MROS3 homologues in A. thaliana, four of them arranged in two tandems, each consisting of two copies. We conclude that MROS3 is a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GV521%2F96%2FK117" target="_blank" >GV521/96/K117: Nové metody pro efektivní studium a mapování genomů kulturních rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genetics resources and crop evolution

ISSN

0925-9864

e-ISSN

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Svazek periodika

N

Číslo periodika v rámci svazku

158

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

1269-1277

Kód UT WoS článku

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EID výsledku v databázi Scopus

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