Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F01%3A00001274" target="_blank" >RIV/61989592:15310/01:00001274 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry.

Popis výsledku v původním jazyce

The analysis of wheat genome could be simplified by its separation into individual chromosome and/or chromosome arms. The aim of this study was to develop an improved procedure for preparation of chromosome suspensions and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenisation of synchronized root tip meristems after a mild formaldehyde fixation. The analysis of DAPI-staining chromosome suspensions resulted in flow karyotypes containing three composite peaks representing groups of chromosomes. In addition, a peak that represented chromosome 3B was well resolved. This chromosome could be sorted at high purity. Sorting of other chromosomes was not possible.The work is in progress to assess the suitability of various chromosome stocks to overcome this problem. For instance using chromosome translocation lines it was possible to sort chromosome 5BL/7BL. Furthermore, some ditelosomic lines wer

Název v anglickém jazyce

Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry.

Popis výsledku anglicky

The analysis of wheat genome could be simplified by its separation into individual chromosome and/or chromosome arms. The aim of this study was to develop an improved procedure for preparation of chromosome suspensions and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenisation of synchronized root tip meristems after a mild formaldehyde fixation. The analysis of DAPI-staining chromosome suspensions resulted in flow karyotypes containing three composite peaks representing groups of chromosomes. In addition, a peak that represented chromosome 3B was well resolved. This chromosome could be sorted at high purity. Sorting of other chromosomes was not possible.The work is in progress to assess the suitability of various chromosome stocks to overcome this problem. For instance using chromosome translocation lines it was possible to sort chromosome 5BL/7BL. Furthermore, some ditelosomic lines wer

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GV521%2F96%2FK117" target="_blank" >GV521/96/K117: Nové metody pro efektivní studium a mapování genomů kulturních rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Bedö, Z., Láng, L. (eds.): Wheat in a Global Environment

ISBN

0-7923-6722-7

Počet stran výsledku

787

Strana od-do

531-536

Počet stran knihy

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Název nakladatele

Kluwer

Místo vydání

Dordrecht

Kód UT WoS kapitoly

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