Light microscopic localization of aminoaldehyde dehydrogenase activity in plant tissues using nitroblue tetrazolium-based staining method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F01%3A00001378" target="_blank" >RIV/61989592:15310/01:00001378 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Light microscopic localization of aminoaldehyde dehydrogenase activity in plant tissues using nitroblue tetrazolium-based staining method

Popis výsledku v původním jazyce

We have recently isolated pea aminoaldehydedehydrogenase(AMADH,EC1.2.1.-)and showed that it oxidises various -aminoaldehydes, but not elementary aldehydes and betaine aldehyde. Now a simple method for specific staining in polyacrylamide gels was optimised enabling us to localise AMADH activity in plant tissues. When phenazine methosulphate (PMS) was used as a mediator, AMADH in native PAGE gels readily reduced thiazolyl blue (MTT) or nitroblue tetrazolium (NBT) producing the corresponding coloured formazans. Using NBT-based staining solution, AMADH activity was localised in cross sections of the root, hypocotyl, epicotyl and shoot apex of 7-d-old etiolated pea seedlings. We followed a histochemical approach for the enzyme localisation in order to visualise tissues where the aminoaldehydes formed by the amine oxidase reaction are probably metabolised. In the root and hypocotyl, activity staining was most intense in cells belonging to the pericycle and endodermis. Weaker staining (namely

Název v anglickém jazyce

Light microscopic localization of aminoaldehyde dehydrogenase activity in plant tissues using nitroblue tetrazolium-based staining method

Popis výsledku anglicky

We have recently isolated pea aminoaldehydedehydrogenase(AMADH,EC1.2.1.-)and showed that it oxidises various -aminoaldehydes, but not elementary aldehydes and betaine aldehyde. Now a simple method for specific staining in polyacrylamide gels was optimised enabling us to localise AMADH activity in plant tissues. When phenazine methosulphate (PMS) was used as a mediator, AMADH in native PAGE gels readily reduced thiazolyl blue (MTT) or nitroblue tetrazolium (NBT) producing the corresponding coloured formazans. Using NBT-based staining solution, AMADH activity was localised in cross sections of the root, hypocotyl, epicotyl and shoot apex of 7-d-old etiolated pea seedlings. We followed a histochemical approach for the enzyme localisation in order to visualise tissues where the aminoaldehydes formed by the amine oxidase reaction are probably metabolised. In the root and hypocotyl, activity staining was most intense in cells belonging to the pericycle and endodermis. Weaker staining (namely

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

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Svazek periodika

N

Číslo periodika v rámci svazku

39

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

9

Strana od-do

831-839

Kód UT WoS článku

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EID výsledku v databázi Scopus

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