Catalytic reaction of cytokinin oxidase/dehydrogenase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F02%3A00001493" target="_blank" >RIV/61989592:15310/02:00001493 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Catalytic reaction of cytokinin oxidase/dehydrogenase

Popis výsledku v původním jazyce

An enzyme, formerly named cytokinin oxidase, specifically degrades all cytokinins of isopentenyl adenine and zeatin type producing adenine and the corresponding side-chain aldehyde. The enzyme is encoded by a small gene family in Arabidopsis and apparently in other species as well, including the monocots maize, rice, barley, and wheat. Recent studies by our group have shown, however, that the enzyme appears unlikely to utilize oxygen but other electron acceptors in the catalytic reaction. Number of artificial electron acceptors have been described, such as phenazine methosufate, 2,6-dichlorophenol-indophenol, methylene blue and 3-dimethoxy-5-methyl-1,4-benzoquinone with E0' values in the range of 0.08-0.22 V. Since the classification as a dehydrogenasebetter conforms with the character of the enzymatic reaction, a new number EC 1.5.99.12 has been assigned to this enzyme that is renamed to cytokinin dehydrogenase (CKX). We present further characterization of the redox catalytic reaction

Název v anglickém jazyce

Catalytic reaction of cytokinin oxidase/dehydrogenase

Popis výsledku anglicky

An enzyme, formerly named cytokinin oxidase, specifically degrades all cytokinins of isopentenyl adenine and zeatin type producing adenine and the corresponding side-chain aldehyde. The enzyme is encoded by a small gene family in Arabidopsis and apparently in other species as well, including the monocots maize, rice, barley, and wheat. Recent studies by our group have shown, however, that the enzyme appears unlikely to utilize oxygen but other electron acceptors in the catalytic reaction. Number of artificial electron acceptors have been described, such as phenazine methosufate, 2,6-dichlorophenol-indophenol, methylene blue and 3-dimethoxy-5-methyl-1,4-benzoquinone with E0' values in the range of 0.08-0.22 V. Since the classification as a dehydrogenasebetter conforms with the character of the enzymatic reaction, a new number EC 1.5.99.12 has been assigned to this enzyme that is renamed to cytokinin dehydrogenase (CKX). We present further characterization of the redox catalytic reaction

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

13th Congres of the Federation of European Societies of Plant Physiology

ISBN

—

ISSN

—

e-ISSN

—

Počet stran výsledku

779

Strana od-do

217

Název nakladatele

FESPP

Místo vydání

Greece

Místo konání akce

Heraklion

Datum konání akce

—

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

—