Genes for cytokinin oxidase/dehydrogenase in cereals

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F02%3A00001499" target="_blank" >RIV/61989592:15310/02:00001499 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genes for cytokinin oxidase/dehydrogenase in cereals

Popis výsledku v původním jazyce

Properties of an enzyme degrading cytokinins with isoprenoic side chain, previously named cytokinin oxidase were described and a new EC number has been preliminary assigned as 1.5.99.12. Cytokinin dehydrogenase is a 60kDa flavoprotein co-operating with some electron acceptor which features and localisation have not been elucidated yet. Enzyme has a crucial position in the metabolism of cytokinins, both endogenous or externally applied to plants. First structural cytokinin oxidase gene, ckx1, has been purified and characterised from maize in 1999. A family of seven genes has been found in Arabidopsis genome with high homology to the ckx1. We suggested several degenerated primers on the base of homology between Zea mays ckx gene and hypothetical genes from Arabidopsis. Poly+mRNA was isolated from barley and wheat grains and used as template for RT-PCR with all combination of primers. Two 413 bp fragments of genes encoding cytokinin dehydrogenase corresponded to protein C-terminus were cl

Název v anglickém jazyce

Genes for cytokinin oxidase/dehydrogenase in cereals

Popis výsledku anglicky

Properties of an enzyme degrading cytokinins with isoprenoic side chain, previously named cytokinin oxidase were described and a new EC number has been preliminary assigned as 1.5.99.12. Cytokinin dehydrogenase is a 60kDa flavoprotein co-operating with some electron acceptor which features and localisation have not been elucidated yet. Enzyme has a crucial position in the metabolism of cytokinins, both endogenous or externally applied to plants. First structural cytokinin oxidase gene, ckx1, has been purified and characterised from maize in 1999. A family of seven genes has been found in Arabidopsis genome with high homology to the ckx1. We suggested several degenerated primers on the base of homology between Zea mays ckx gene and hypothetical genes from Arabidopsis. Poly+mRNA was isolated from barley and wheat grains and used as template for RT-PCR with all combination of primers. Two 413 bp fragments of genes encoding cytokinin dehydrogenase corresponded to protein C-terminus were cl

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

13th Congres of the Federation of European Societies of Plant Physiology

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

779

Strana od-do

218

Název nakladatele

FESPP

Místo vydání

Greece

Místo konání akce

Heraklion

Datum konání akce

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Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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