Superoxid nebo singletový kyslík: chemiluminiscence analogů luciferinu Cypridiny ve fotodynamických roztocích

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002044" target="_blank" >RIV/61989592:15310/04:00002044 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Superoxide or singlet oxygen: The chemiluminescence of Cypridina luciferin analogues in photodynamic solutions the chemiluminecent measurement of the black and green tea antioxidant capacity and the comparision with their antimicrobial activity

Popis výsledku v původním jazyce

Luminol, CLA and MCLA CL were studied in three ROS generating chemical systems in vitro (HRP - H2O2, CuSO4 - H2O2 and NaClO - H2O2 system). Measurements were carried out in phosphate buffer (pH 7.4) at 25°C and 37°C using luminometer Fluoroskan Ascent FL. Superoxide dismutase and sodium azide were used as specific scavenger of superoxide anion radical and quencher of singlet oxygen, respectively. Their effect was compared with that of Trolox (water soluble analogue of vitamin E), which should scavenge all reactive species present in the reaction mixture, and tryptophan. As the photodynamic systems we used e.g. phthalocyanines and chlorophyll a. The declared specifity of Cypridina luciferin analogues chemiluminescence based on superoxide and singlet oxygen and the suitability of this prove is discussed.

Název v anglickém jazyce

Superoxide or singlet oxygen: The chemiluminescence of Cypridina luciferin analogues in photodynamic solutions the chemiluminecent measurement of the black and green tea antioxidant capacity and the comparision with their antimicrobial activity

Popis výsledku anglicky

Luminol, CLA and MCLA CL were studied in three ROS generating chemical systems in vitro (HRP - H2O2, CuSO4 - H2O2 and NaClO - H2O2 system). Measurements were carried out in phosphate buffer (pH 7.4) at 25°C and 37°C using luminometer Fluoroskan Ascent FL. Superoxide dismutase and sodium azide were used as specific scavenger of superoxide anion radical and quencher of singlet oxygen, respectively. Their effect was compared with that of Trolox (water soluble analogue of vitamin E), which should scavenge all reactive species present in the reaction mixture, and tryptophan. As the photodynamic systems we used e.g. phthalocyanines and chlorophyll a. The declared specifity of Cypridina luciferin analogues chemiluminescence based on superoxide and singlet oxygen and the suitability of this prove is discussed.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Luminescence

ISSN

1522-7235

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

1

Strana od-do

133

Kód UT WoS článku

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EID výsledku v databázi Scopus

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