Analytická derivatizace - nástroj pro stanovení kyseliny orotové

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002132" target="_blank" >RIV/61989592:15310/04:00002132 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/04:00000802 RIV/61989592:15110/04:00001902

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analytical derivatization - a tool for determination of orotic acid

Popis výsledku v původním jazyce

Derivatization of orotic acid (OA) into various forms (ttimethylsilylderivate, alkyl ester and per-methylated derivate) and their evaluation by GUMS is described. The tested approach includes ion-exchange SPE clean-up, evaporation and chemical reaction with different types of derivatization agents (N,O-bis-(trimethylsilyl)trifluoroacetamide with trimethylehlorosilane, butanol with acetylchloride and ethereal solution of diazomethane). Derivate originated in the reaction with diazomethane was used for determination of urinary orotic acid by GC/MS. Detection limit of 0.28 mumol l(-1) was reached using the ion 82 m/z in single ion monitoring (SIM) mode. Linearity of the method was tested within the range of 3.4-2503.4 mumol l(-1) covering physiological and pathological levels of orotic acid in urine sample. Recoveries were within the range 93.7-110.6%. Application of the method on the patient with defect of ornithine transcarbamylase (OTC) was demonstrated as well. (C) 2003 Elsevier B.V.

Název v anglickém jazyce

Analytical derivatization - a tool for determination of orotic acid

Popis výsledku anglicky

Derivatization of orotic acid (OA) into various forms (ttimethylsilylderivate, alkyl ester and per-methylated derivate) and their evaluation by GUMS is described. The tested approach includes ion-exchange SPE clean-up, evaporation and chemical reaction with different types of derivatization agents (N,O-bis-(trimethylsilyl)trifluoroacetamide with trimethylehlorosilane, butanol with acetylchloride and ethereal solution of diazomethane). Derivate originated in the reaction with diazomethane was used for determination of urinary orotic acid by GC/MS. Detection limit of 0.28 mumol l(-1) was reached using the ion 82 m/z in single ion monitoring (SIM) mode. Linearity of the method was tested within the range of 3.4-2503.4 mumol l(-1) covering physiological and pathological levels of orotic acid in urine sample. Recoveries were within the range 93.7-110.6%. Application of the method on the patient with defect of ornithine transcarbamylase (OTC) was demonstrated as well. (C) 2003 Elsevier B.V.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GP303%2F02%2FD010" target="_blank" >GP303/02/D010: Aplikace kapilární elektroforézy pro analýzu biologických materiálů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

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Svazek periodika

799

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

303-309

Kód UT WoS článku

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EID výsledku v databázi Scopus

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