Místně cílená mutageneze cytokinindehydrogenasového genu AtCKX2 z Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002239" target="_blank" >RIV/61989592:15310/04:00002239 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Site-directed mutagenesis of the cytokinin dehydrogenase gene AtCKX2 from Arabidopsis thaliana

Popis výsledku v původním jazyce

Cell division, shoot development, growth of lateral buds, photosynthesis, chloroplast development, and delay of leaf senescence1 are processes in plants that are regulated by hormones cytokinins. A level of these hormones is regulated by their natural degradation catalyzed by the enzyme cytokinin dehydrogenase (CKX; EC 1.5.99.12). N6-chain cleavage of cytokinins2 occurs by a dehydrogenation mechanism in the active site of the CKX enzyme. Biochemical experiments show that the enzyme mediates this reaction by means of the covalently bound FAD cofactor that forms ternary complex with cytokinin substrate and electron acceptor3,4. In a majority of plants, cytokinin dehydrogenase is present in several gene forms. Multiple sequence alignment of all known CKXsreveals that they share high sequence homology in the FAD-binding and the C-terminal domains. FAD binding domain contains highly conserved N-terminal motif GHS where His is the FAD-binding residue. C-terminus of CKXs contains the motifs

Název v anglickém jazyce

Site-directed mutagenesis of the cytokinin dehydrogenase gene AtCKX2 from Arabidopsis thaliana

Popis výsledku anglicky

Cell division, shoot development, growth of lateral buds, photosynthesis, chloroplast development, and delay of leaf senescence1 are processes in plants that are regulated by hormones cytokinins. A level of these hormones is regulated by their natural degradation catalyzed by the enzyme cytokinin dehydrogenase (CKX; EC 1.5.99.12). N6-chain cleavage of cytokinins2 occurs by a dehydrogenation mechanism in the active site of the CKX enzyme. Biochemical experiments show that the enzyme mediates this reaction by means of the covalently bound FAD cofactor that forms ternary complex with cytokinin substrate and electron acceptor3,4. In a majority of plants, cytokinin dehydrogenase is present in several gene forms. Multiple sequence alignment of all known CKXsreveals that they share high sequence homology in the FAD-binding and the C-terminal domains. FAD binding domain contains highly conserved N-terminal motif GHS where His is the FAD-binding residue. C-terminus of CKXs contains the motifs

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organizace genů cytokinin dehydrogenázy v obilovinách a mechanismus katalytické reakce odbourávání cytokininů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica

ISSN

0232-0061

e-ISSN

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Svazek periodika

43

Číslo periodika v rámci svazku

S

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

275

Strana od-do

257-258

Kód UT WoS článku

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EID výsledku v databázi Scopus

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