Dva cytokininové receptory v Arabidopsis thaliana, CRE1/AHK4 a AHK3, se liší ve své ligandové specifitě v bakteriálním testu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002272" target="_blank" >RIV/61989592:15310/04:00002272 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Two Cytokinin Receptors of Arabidopsis, CRE1/AHK4 and AHK3, Differ in Their Ligand Specifity in a Bacterial Assay

Popis výsledku v původním jazyce

Strains of Escherichia coli that express two different cytokinin receptors of Arabidopsis thaliana, CRE1/AHK4 and AHK3, were used to study the relative sensitivity of these receptors to different cytokinin compounds. CRE1/AHK4 recognized only trans-zeatin while AHK3 recognized also cis-zeatin and dihydrozeatin, although with a lower sensitivity. In addition, AHK3 was activated by cytokinin ribosides and ribotides, which did not activate CRE1/AHK4. Comparisons using the ARR5::GUS fusion gene as a cytokinin reporter in Arabidopsis showed similar relative degrees of responses in planta, with the exception that aromatic cytokinins showed much higher activities than in the bacterial assay. These results indicate that the different local concentration of thediverse cytokinin metabolites might have specific functions in the numerous cytokinin-regulated processes, which may depend in turn on different receptors and their associated signalling pathways.

Název v anglickém jazyce

Two Cytokinin Receptors of Arabidopsis, CRE1/AHK4 and AHK3, Differ in Their Ligand Specifity in a Bacterial Assay

Popis výsledku anglicky

Strains of Escherichia coli that express two different cytokinin receptors of Arabidopsis thaliana, CRE1/AHK4 and AHK3, were used to study the relative sensitivity of these receptors to different cytokinin compounds. CRE1/AHK4 recognized only trans-zeatin while AHK3 recognized also cis-zeatin and dihydrozeatin, although with a lower sensitivity. In addition, AHK3 was activated by cytokinin ribosides and ribotides, which did not activate CRE1/AHK4. Comparisons using the ARR5::GUS fusion gene as a cytokinin reporter in Arabidopsis showed similar relative degrees of responses in planta, with the exception that aromatic cytokinins showed much higher activities than in the bacterial assay. These results indicate that the different local concentration of thediverse cytokinin metabolites might have specific functions in the numerous cytokinin-regulated processes, which may depend in turn on different receptors and their associated signalling pathways.

Druh

D - Stať ve sborníku

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

18th IPGSA conference, Conference Handbook

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

144

Strana od-do

93

Název nakladatele

Australian National University

Místo vydání

Canberra

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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