Imunotesty na kyselinu abcisovou (ABA)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002314" target="_blank" >RIV/61989592:15310/04:00002314 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immunoassays of Abcisic Acid (ABA)

Popis výsledku v původním jazyce

Immunoassay for abscisic acid (ABA) has rapidly developed since 1979 and has been confirmed as the most sensitive and selective detection method for ABA. Use of immunoassay is most highly recommended for quantification of ABA and related compounds. Abscisic acid, like indole acetic acid, has two easily accessible sites for conjugation to protein carriers. Most of the ABA antibodies that have been produced have been raised against conjugates formed by amide bond formation at the C1 carboxyl group using either carbodiimide or mixed anhydride coupling reactions. Antigen conjugated to C-1 of ABA through an amide bond is used for total ABA determination. In order to have ABA specific antibodies two procedures based on aromatic hydrazones have been developed. Initially, Weiler (1980) prepared racemic ABA-4´-tyrosylhydrazone and coupled it to BSA, which had been prederivatised with p-aminohippuric acid, by a diazo-coupling reaction. Couplig at C-4´was simplified by Quarrie and Galfre (1985) w

Název v anglickém jazyce

Immunoassays of Abcisic Acid (ABA)

Popis výsledku anglicky

Immunoassay for abscisic acid (ABA) has rapidly developed since 1979 and has been confirmed as the most sensitive and selective detection method for ABA. Use of immunoassay is most highly recommended for quantification of ABA and related compounds. Abscisic acid, like indole acetic acid, has two easily accessible sites for conjugation to protein carriers. Most of the ABA antibodies that have been produced have been raised against conjugates formed by amide bond formation at the C1 carboxyl group using either carbodiimide or mixed anhydride coupling reactions. Antigen conjugated to C-1 of ABA through an amide bond is used for total ABA determination. In order to have ABA specific antibodies two procedures based on aromatic hydrazones have been developed. Initially, Weiler (1980) prepared racemic ABA-4´-tyrosylhydrazone and coupled it to BSA, which had been prederivatised with p-aminohippuric acid, by a diazo-coupling reaction. Couplig at C-4´was simplified by Quarrie and Galfre (1985) w

Druh

D - Stať ve sborníku

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/IBS5038351" target="_blank" >IBS5038351: Imunodiagnostika přírodních látek hormonální povahy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

10th Days of Plant Physiology

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

136

Strana od-do

44

Název nakladatele

Univerzita Komenského

Místo vydání

Bratislava

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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