První detekce fytoplazem u rododendronu v České Republice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002421" target="_blank" >RIV/61989592:15310/04:00002421 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

First Detection of Phytoplasmas in Rhododendron in the Czech Republic

Popis výsledku v původním jazyce

A 10-year-old plant of rhododendron (#Cunningham#s White# PS986) with leaf malformation and variegation was observed in Prague in 1997. This plant was micropropagated, and regenerants with severe, mild and no symptoms were obtained. Phytoplasma infectionwas detected using nested polymerase chain reaction (PCR) in the original PS986 plant and the symptomatic regenerants but not in nonsymptomatic plants. During 2002, phytoplasmas were also detected in rhododendron hybrids, PS2716 and PS2439, grown in Pruhonice that showed similar symptoms as the plant observed in Prague. In nested PCR, performed as described previously (1), primer pairs R16F1/R16R0 and R16F2/R16R2 were used. The phytoplasmas detected were classified by restriction fragment length polymorphism analysis using R16F2/R16R2 PCR fragments. Following digestion with restriction endonuclease MseI (Promega, Madison, WI), the restriction profiles obtained were identical with the pattern of the stolbur phytoplasma group (16SrXII gr

Název v anglickém jazyce

First Detection of Phytoplasmas in Rhododendron in the Czech Republic

Popis výsledku anglicky

A 10-year-old plant of rhododendron (#Cunningham#s White# PS986) with leaf malformation and variegation was observed in Prague in 1997. This plant was micropropagated, and regenerants with severe, mild and no symptoms were obtained. Phytoplasma infectionwas detected using nested polymerase chain reaction (PCR) in the original PS986 plant and the symptomatic regenerants but not in nonsymptomatic plants. During 2002, phytoplasmas were also detected in rhododendron hybrids, PS2716 and PS2439, grown in Pruhonice that showed similar symptoms as the plant observed in Prague. In nested PCR, performed as described previously (1), primer pairs R16F1/R16R0 and R16F2/R16R2 were used. The phytoplasmas detected were classified by restriction fragment length polymorphism analysis using R16F2/R16R2 PCR fragments. Following digestion with restriction endonuclease MseI (Promega, Madison, WI), the restriction profiles obtained were identical with the pattern of the stolbur phytoplasma group (16SrXII gr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F98%2F0792" target="_blank" >GA522/98/0792: Biologická a genetická charakteristika fytoplazem vyskytujících se na území České republiky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Disease

ISSN

0191-2917

e-ISSN

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Svazek periodika

88

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

1

Strana od-do

906-906

Kód UT WoS článku

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EID výsledku v databázi Scopus

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