CD4(+)-T-cell responses generated during murine Salmonella enterica serovar typhimurium infection are directed towards multiple epitopes within the natural antigen FliC

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F05%3A00010746" target="_blank" >RIV/61989592:15310/05:00010746 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

CD4(+)-T-cell responses generated during murine Salmonella enterica serovar typhimurium infection are directed towards multiple epitopes within the natural antigen FliC

Popis výsledku v původním jazyce

The flagellar filament protein FliC is a natural antigen recognized by memory CD4+-T cells recovered from Salmonella enterica serovar Typhimurium-infected humans and mice. To further investigate T-cell response to FliC, we derived FliC-specific CD4+-T-cell clones from mice of two different haplotypes following oral S. enterica serovar Typhimurium infection. Using C-terminal truncations of MalE-FliC recombinant vision proteins, we mapped antigenic activity to four different regions of FliC; three of thefour epitope-containing regions were present in both FliC and the alternate flagellin subunit FljB. We determined that two novel FliC epitopes were also present in flagellins from several gram-negative enteric bacterial species: Ek-restricted FliC 80?94(amino acids 80 to 94) and Ab-restricted FliC 455?469. Further mapping confirmed the presence of two previously identified FliC epitopes: Ak-restricted FliC 339?350 and Ab-restricted FliC 428?442. Therefore, like the recognition site of t

Název v anglickém jazyce

CD4(+)-T-cell responses generated during murine Salmonella enterica serovar typhimurium infection are directed towards multiple epitopes within the natural antigen FliC

Popis výsledku anglicky

The flagellar filament protein FliC is a natural antigen recognized by memory CD4+-T cells recovered from Salmonella enterica serovar Typhimurium-infected humans and mice. To further investigate T-cell response to FliC, we derived FliC-specific CD4+-T-cell clones from mice of two different haplotypes following oral S. enterica serovar Typhimurium infection. Using C-terminal truncations of MalE-FliC recombinant vision proteins, we mapped antigenic activity to four different regions of FliC; three of thefour epitope-containing regions were present in both FliC and the alternate flagellin subunit FljB. We determined that two novel FliC epitopes were also present in flagellins from several gram-negative enteric bacterial species: Ek-restricted FliC 80?94(amino acids 80 to 94) and Ab-restricted FliC 455?469. Further mapping confirmed the presence of two previously identified FliC epitopes: Ak-restricted FliC 339?350 and Ab-restricted FliC 428?442. Therefore, like the recognition site of t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Infection and Immunity

ISSN

0019-9567

e-ISSN

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Svazek periodika

73

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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