Zkoumání homeostázy v A. thaliana konstitutivní nadměrnou expresí dvou forem genu pro kukuřičnou cytokininoxidasu/dehydrogenasu 1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00002797" target="_blank" >RIV/61989592:15310/06:00002797 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/06:00083205

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene

Popis výsledku v původním jazyce

Engineering transgenic plantswith reduced cytokinin (Ck) contents is away to analyze the role of these hormones in growth and development. Cytokinin oxidase/dehydrogenase (CKO) genes are good candidates to promote Ck deficiency. They code for enzymes degrading Cks and generally belong to multigene families. Plants constitutively expressing naturally occurring CKO genes that code for secreted or vacuolar enzymes have been described.We report on Arabidopsis transgenics constitutively overexpressing the secreted native formor an engineered nonsecreted form of the maize CKO1 enzyme. Severity of phenotype symptoms (increased root system, reduced size of aerial parts and defects in seed development) was clearly correlated with the level of enzyme activity. Aerial part was especially affected in plants overexpressing the nonsecreted enzyme, even at low activity level. In all strong overexpressers, zeatin-type metabolites were highly depleted compared to isopentenyladenine-type metabolites. At

Název v anglickém jazyce

Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene

Popis výsledku anglicky

Engineering transgenic plantswith reduced cytokinin (Ck) contents is away to analyze the role of these hormones in growth and development. Cytokinin oxidase/dehydrogenase (CKO) genes are good candidates to promote Ck deficiency. They code for enzymes degrading Cks and generally belong to multigene families. Plants constitutively expressing naturally occurring CKO genes that code for secreted or vacuolar enzymes have been described.We report on Arabidopsis transgenics constitutively overexpressing the secreted native formor an engineered nonsecreted form of the maize CKO1 enzyme. Severity of phenotype symptoms (increased root system, reduced size of aerial parts and defects in seed development) was clearly correlated with the level of enzyme activity. Aerial part was especially affected in plants overexpressing the nonsecreted enzyme, even at low activity level. In all strong overexpressers, zeatin-type metabolites were highly depleted compared to isopentenyladenine-type metabolites. At

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Science

ISSN

0168-9452

e-ISSN

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Svazek periodika

171

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

114-122

Kód UT WoS článku

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EID výsledku v databázi Scopus

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