Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003084" target="_blank" >RIV/61989592:15310/06:00003084 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/06:00081794

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)

Popis výsledku v původním jazyce

Musa spp ( bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. This study determined the ploidy levels and genome composition of the Musa germplasm collection, constituting over 300 accessions, at the International Institute of Tropical Agriculture in Nigeria and Uganda. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels, while genome composition was ascertained with RAPD markers that are specific for the A and B genomes of Musa. It was determined that at least 8% of the plants in thegermplasm collection were miss-classified in terms of ploidy and/or genome composition. The cultivars 'Pisang awak', 'Foulah 4' and 'Nzizi', previously classified as triploids, were found to be tetraploids by flow cytometry

Název v anglickém jazyce

Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)

Popis výsledku anglicky

Musa spp ( bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. This study determined the ploidy levels and genome composition of the Musa germplasm collection, constituting over 300 accessions, at the International Institute of Tropical Agriculture in Nigeria and Uganda. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels, while genome composition was ascertained with RAPD markers that are specific for the A and B genomes of Musa. It was determined that at least 8% of the plants in thegermplasm collection were miss-classified in terms of ploidy and/or genome composition. The cultivars 'Pisang awak', 'Foulah 4' and 'Nzizi', previously classified as triploids, were found to be tetraploids by flow cytometry

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06004" target="_blank" >LC06004: Integrovaný výzkum rostlinného genomu</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

African Journal of Biotechnology

ISSN

1684-5315

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

13

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1224-1232

Kód UT WoS článku

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EID výsledku v databázi Scopus

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