Kapilární elektroforéza jako ověřovací nástroj pro imunochemický screening drog

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004826" target="_blank" >RIV/61989592:15310/07:00004826 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Capillary electrophoresis as a verification tool for immunochemical drug screening

Popis výsledku v původním jazyce

Background: The aim of this work was to develop a simple capillary electrophoretic method as the verifi cation and confi rmation tool in the screening analysis for amphetamines, opiates, benzodiazepines and cocaine and their metabolites for toxicologicalapplications. Methods: 50 mM phosphate Tris pH 2.0 with 30 % (v/v) of methanol was used as a background electrolyte that enabled fast separation of drugs and their metabolites in saliva and urine. Verifi cation of the data from the electrophoretic method was done by High Performance Thin Layer Chromatography (HPTLC) and the immunochemical screening test QuikScreen. Results: The experimental conditions of the Capillary Electrophoresis (CE) were partially optimized (mainly the infl uence of concentrationand types of additives, e.g. cyclodextrines, organic solvents) and validated; the method was used for analysing samples from drug abusers. Conclusions: The non-instrumental, immunoassay tests could only confi rm qualitative addictions an

Název v anglickém jazyce

Capillary electrophoresis as a verification tool for immunochemical drug screening

Popis výsledku anglicky

Background: The aim of this work was to develop a simple capillary electrophoretic method as the verifi cation and confi rmation tool in the screening analysis for amphetamines, opiates, benzodiazepines and cocaine and their metabolites for toxicologicalapplications. Methods: 50 mM phosphate Tris pH 2.0 with 30 % (v/v) of methanol was used as a background electrolyte that enabled fast separation of drugs and their metabolites in saliva and urine. Verifi cation of the data from the electrophoretic method was done by High Performance Thin Layer Chromatography (HPTLC) and the immunochemical screening test QuikScreen. Results: The experimental conditions of the Capillary Electrophoresis (CE) were partially optimized (mainly the infl uence of concentrationand types of additives, e.g. cyclodextrines, organic solvents) and validated; the method was used for analysing samples from drug abusers. Conclusions: The non-instrumental, immunoassay tests could only confi rm qualitative addictions an

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Svazek periodika

151

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

31-36

Kód UT WoS článku

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EID výsledku v databázi Scopus

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