Metody izolace pylových zrn Cucumis sativus a C. melo a jejich využití v in vitro opylování

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005259" target="_blank" >RIV/61989592:15310/08:00005259 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Methods of isolation of Cucumis sativus and C. melo pollen grains and their utilization in in vitro pollination

Popis výsledku v původním jazyce

Optimizing manipulation with pollen grains is necessary for successful in vitro pollination. This pollination can be used for overcoming crossing barriers in interspecific hybridization in the genus Cucumis. Pollen grains from Cucumis sativus and C. melowere isolated by various methods. Different techniques of centrifugation were compared with direct isolation of pollen grains from anthers. The viability of pollen grains was evaluated and their ability to form pollen tubes was tested. Three isolation media (NLN, YST and VB) and two cultivation media (YS and CP) were used. Then pollen grains were cultivated with ovules of C. sativus and the level of regeneration was observed. The growing and green ovules were considered as the lowest level and the highest level was the formation of calli.

Název v anglickém jazyce

Methods of isolation of Cucumis sativus and C. melo pollen grains and their utilization in in vitro pollination

Popis výsledku anglicky

Optimizing manipulation with pollen grains is necessary for successful in vitro pollination. This pollination can be used for overcoming crossing barriers in interspecific hybridization in the genus Cucumis. Pollen grains from Cucumis sativus and C. melowere isolated by various methods. Different techniques of centrifugation were compared with direct isolation of pollen grains from anthers. The viability of pollen grains was evaluated and their ability to form pollen tubes was tested. Three isolation media (NLN, YST and VB) and two cultivation media (YS and CP) were used. Then pollen grains were cultivated with ovules of C. sativus and the level of regeneration was observed. The growing and green ovules were considered as the lowest level and the highest level was the formation of calli.

Druh

D - Stať ve sborníku

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QF4108" target="_blank" >QF4108: Využití techniky fúze protoplastů ve šlechtění hospodářsky významných plodin rodu Brassica, Cucumis a Solanum</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Cucurbitaceae 2008: Proceedings of the IXth EUCARPIA Meeting on Genetics and Breeding Cucurbitaceae

ISBN

978-2-7380-1251-7

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

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Název nakladatele

INRA

Místo vydání

Avignon, France

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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