Energetics, conformation, and recognition of DNA duplexes modified by methylated analogues of [PtCl(dien)]+. Chem Eur J, 15, 6211-6221.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010123" target="_blank" >RIV/61989592:15310/09:00010123 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Energetics, conformation, and recognition of DNA duplexes modified by methylated analogues of [PtCl(dien)]+. Chem Eur J, 15, 6211-6221.

Popis výsledku v původním jazyce

We recently synthesized and characterized water-soluble dinuclear RuII arene complexes, in which two {(6-p-isopropyltoluene)RuCl[3-(oxo-O)-2-methyl-4-pyridinonato-O4]} units were linked by flexible chains of different length [(CH2)n (n = 4, 6, 8, 12)]. These new dinuclear ruthenium drugs were found to exert promising cytotoxic effects in human cancer cells. In the present work DNA modifications by these new dinuclear RuII arene compounds, which differed in the length of the linker between the two RuII centers, were examined by biochemical and biophysical methods. The complexes bind DNA forming intrastrand and interstrand cross-links in one DNA molecule in the absence of proteins. An intriguing aspect of the DNA-binding mode of these dinuclear RuII compounds is that they can crosslink two DNA duplexes and also proteins to DNA?a feature not observed for other antitumor ruthenium complexes. Thus, the concept for the design of interhelical and DNA? protein cross-linking agents based on din

Název v anglickém jazyce

Energetics, conformation, and recognition of DNA duplexes modified by methylated analogues of [PtCl(dien)]+. Chem Eur J, 15, 6211-6221.

Popis výsledku anglicky

We recently synthesized and characterized water-soluble dinuclear RuII arene complexes, in which two {(6-p-isopropyltoluene)RuCl[3-(oxo-O)-2-methyl-4-pyridinonato-O4]} units were linked by flexible chains of different length [(CH2)n (n = 4, 6, 8, 12)]. These new dinuclear ruthenium drugs were found to exert promising cytotoxic effects in human cancer cells. In the present work DNA modifications by these new dinuclear RuII arene compounds, which differed in the length of the linker between the two RuII centers, were examined by biochemical and biophysical methods. The complexes bind DNA forming intrastrand and interstrand cross-links in one DNA molecule in the absence of proteins. An intriguing aspect of the DNA-binding mode of these dinuclear RuII compounds is that they can crosslink two DNA duplexes and also proteins to DNA?a feature not observed for other antitumor ruthenium complexes. Thus, the concept for the design of interhelical and DNA? protein cross-linking agents based on din

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/OC09018" target="_blank" >OC09018: Mechanismus účinku protinádorově účinných metalofarmak. Replikace DNA a oprava poškození DNA</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemistry: A European Journal

ISSN

0947-6539

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

25

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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