Micropropagation of wild service tree (Sorbus torminalis [L.] Crantz): The regulative role of different aromatic cytokinins during organogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010228" target="_blank" >RIV/61989592:15310/09:00010228 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/09:00337010 RIV/00020702:_____/09:0R000405

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Micropropagation of wild service tree (Sorbus torminalis [L.] Crantz): The regulative role of different aromatic cytokinins during organogenesis

Popis výsledku v původním jazyce

The influences of three different aromatic cytokinin on in vitro multiplication and rhizogenesis of the wild service tree (Sorbus torminalis [L.] Crantz) were compared. The highest micropropagation was achieved on media containing BAP. On the other hand,the best rooting microcuttings were multiplied on a medium containing MeoBAPR. To compare these results with the levels of endogenous cytokinins, a newly developed UPLC-MS/MS method was used to determine 50 cytokinin metabolites in explants. The concentration of BAP9G, an important metabolite suspected to be responsible for inhibition of rooting and acclimatization problems, was found to be the highest in microcuttings grown on BAP. This agrees well with the results of rooting experiment. Levels of themost active endogenous isoprenoid cytokinins were also suppressed in these explants. This may be the result of a very high BAP uptake into the explants grown on this cytokinin. Differences in the production of ethylene and free IAA were

Název v anglickém jazyce

Micropropagation of wild service tree (Sorbus torminalis [L.] Crantz): The regulative role of different aromatic cytokinins during organogenesis

Popis výsledku anglicky

The influences of three different aromatic cytokinin on in vitro multiplication and rhizogenesis of the wild service tree (Sorbus torminalis [L.] Crantz) were compared. The highest micropropagation was achieved on media containing BAP. On the other hand,the best rooting microcuttings were multiplied on a medium containing MeoBAPR. To compare these results with the levels of endogenous cytokinins, a newly developed UPLC-MS/MS method was used to determine 50 cytokinin metabolites in explants. The concentration of BAP9G, an important metabolite suspected to be responsible for inhibition of rooting and acclimatization problems, was found to be the highest in microcuttings grown on BAP. This agrees well with the results of rooting experiment. Levels of themost active endogenous isoprenoid cytokinins were also suppressed in these explants. This may be the result of a very high BAP uptake into the explants grown on this cytokinin. Differences in the production of ethylene and free IAA were

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Plant Growth Regulation

ISSN

0721-7595

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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