Detecting hydrogen peroxide in leaves in vivo - a comparison of methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010231" target="_blank" >RIV/61989592:15310/09:00010231 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detecting hydrogen peroxide in leaves in vivo - a comparison of methods

Popis výsledku v původním jazyce

Four hydrogen peroxide detecting probes, 3,3-diaminobenzidine (DAB), Amplex Red (AR), Amplex Ultra Red (AUR) and a europium-tetracycline complex (Eu3Tc) were infiltrated into tobacco leaves and tested for sensitivity to light, toxicity, subcellular localization and capacity to detect H2O2 in vivo. In the absence of leaves, in water solutions, AUR was very much sensitive to strong light, AR showed slight light sensitivity, while DAB and Eu3Tc were insensitive to irradiation. When infiltrated into the leaves, the probes decreased the photochemical yield (FPSII) in the following order of effect AR > DAB >AUR > Eu3Tc. With the exception of Eu3Tc, all probes stimulated the build-up of non-photochemical quenching either temporally (DAB, AUR) or permanently (AR), showing that their presence may already limit the photosynthetic capacity of leaves, even in the absence of additional stress. This should be taken into account when using these probes in plant stress experiments. Confocal laser scan

Název v anglickém jazyce

Detecting hydrogen peroxide in leaves in vivo - a comparison of methods

Popis výsledku anglicky

Four hydrogen peroxide detecting probes, 3,3-diaminobenzidine (DAB), Amplex Red (AR), Amplex Ultra Red (AUR) and a europium-tetracycline complex (Eu3Tc) were infiltrated into tobacco leaves and tested for sensitivity to light, toxicity, subcellular localization and capacity to detect H2O2 in vivo. In the absence of leaves, in water solutions, AUR was very much sensitive to strong light, AR showed slight light sensitivity, while DAB and Eu3Tc were insensitive to irradiation. When infiltrated into the leaves, the probes decreased the photochemical yield (FPSII) in the following order of effect AR > DAB >AUR > Eu3Tc. With the exception of Eu3Tc, all probes stimulated the build-up of non-photochemical quenching either temporally (DAB, AUR) or permanently (AR), showing that their presence may already limit the photosynthetic capacity of leaves, even in the absence of additional stress. This should be taken into account when using these probes in plant stress experiments. Confocal laser scan

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiologia Plantarum

ISSN

0031-9317

e-ISSN

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Svazek periodika

135

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

18

Strana od-do

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Kód UT WoS článku

000261717800001

EID výsledku v databázi Scopus

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