High Throughput Multiplexed Method for Evaluation of Enantioselective Performance of Chiral Selectors by HPLC-ESI-MS and Dynamic Titration: Cinchona Alkaloid Carbamates Discriminating N-blocked Amino Acids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010417" target="_blank" >RIV/61989592:15310/09:00010417 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

High Throughput Multiplexed Method for Evaluation of Enantioselective Performance of Chiral Selectors by HPLC-ESI-MS and Dynamic Titration: Cinchona Alkaloid Carbamates Discriminating N-blocked Amino Acids

Popis výsledku v původním jazyce

Because of the generally different interaction of enantiomers with biological systems, there has been an ever increasing demand for artificial highly enantioselective systems that can facilitate separation processes involved in the research and development of enantiomerically pure drugs. Such systems may be discovered by large-scale screening of compound libraries which warrants rapid and cost-efficient screening methods. Here, we demonstrate enantioselectivity determination for systems of cinchona alkaloid carbamates and N-blocked amino acids using HPLC-MS and the recently developed dynamic titration technique (Frycak P, Schug KA. Anal Chem 2008;80:13851393). A mixture of nine N-blocked amino acids (either D or L enantiomers) was separated on a reversed-phase column with cinchona alkaloid carbamates added post-column. Dissociation constants of the observed noncovalent complexes were determined from the HPLC-MS data. Enantioselectivity was then calculated from the dissociation constant

Název v anglickém jazyce

High Throughput Multiplexed Method for Evaluation of Enantioselective Performance of Chiral Selectors by HPLC-ESI-MS and Dynamic Titration: Cinchona Alkaloid Carbamates Discriminating N-blocked Amino Acids

Popis výsledku anglicky

Because of the generally different interaction of enantiomers with biological systems, there has been an ever increasing demand for artificial highly enantioselective systems that can facilitate separation processes involved in the research and development of enantiomerically pure drugs. Such systems may be discovered by large-scale screening of compound libraries which warrants rapid and cost-efficient screening methods. Here, we demonstrate enantioselectivity determination for systems of cinchona alkaloid carbamates and N-blocked amino acids using HPLC-MS and the recently developed dynamic titration technique (Frycak P, Schug KA. Anal Chem 2008;80:13851393). A mixture of nine N-blocked amino acids (either D or L enantiomers) was separated on a reversed-phase column with cinchona alkaloid carbamates added post-column. Dissociation constants of the observed noncovalent complexes were determined from the HPLC-MS data. Enantioselectivity was then calculated from the dissociation constant

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chirality

ISSN

0899-0042

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—