Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010605" target="_blank" >RIV/61989592:15310/09:00010605 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility

Popis výsledku v původním jazyce

We investigated the effectiveness of short mitochondrial DNA fragments for the identification of lycid larvae. The rrnL, cox1 and nad5 mtDNA sequences from 17 specimens of immature stages of Lycidae and Lampyridae were combined with a previously published dataset of homologous fragments representing all major lineages of Lycidae and outgroups. Their relationships were analysed under parsimony criteria. We demonstrate that high-density profiles are necessary for accurate identification of unknown samplesto generic and tribal levels and that a multilocus approach is critical for obtaining reliable results. Although widely used, the cox1 mtDNA fragment showed the worst performance for identification at genus level when the query species was not present in the library. Stronger support for deeper branches came from rrnL mtDNA. The neotenic female larvae and male adult stages of Platerodrilus sp. and Macrolibnetis depressus Pic, 1938 were associated by mtDNA fragments. Based on the present

Název v anglickém jazyce

Identification of net-winged beetle larvae (Coleoptera: Lycidae) using three mtDNA fragments: a comparison of their utility

Popis výsledku anglicky

We investigated the effectiveness of short mitochondrial DNA fragments for the identification of lycid larvae. The rrnL, cox1 and nad5 mtDNA sequences from 17 specimens of immature stages of Lycidae and Lampyridae were combined with a previously published dataset of homologous fragments representing all major lineages of Lycidae and outgroups. Their relationships were analysed under parsimony criteria. We demonstrate that high-density profiles are necessary for accurate identification of unknown samplesto generic and tribal levels and that a multilocus approach is critical for obtaining reliable results. Although widely used, the cox1 mtDNA fragment showed the worst performance for identification at genus level when the query species was not present in the library. Stronger support for deeper branches came from rrnL mtDNA. The neotenic female larvae and male adult stages of Platerodrilus sp. and Macrolibnetis depressus Pic, 1938 were associated by mtDNA fragments. Based on the present

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA206%2F06%2F1392" target="_blank" >GA206/06/1392: Neotenie a speciace u Elateroidea</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Systematic Entomology

ISSN

0307-6970

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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