Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010627" target="_blank" >RIV/61989592:15310/09:00010627 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61389030:_____/09:00328264
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium
Popis výsledku v původním jazyce
Various experiments were conducted to characterize and control factors affecting shoot-tip necrosis (STN) in Harpagophytum procumbens. Higher cytokinin concentrations increased the incidence of STN and the problem was aggravated by the addition of auxin(IAA) to the multiplication medium. Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta-Topolin riboside (mTR). In the presence of auxin, plantlets produced basal callus that interfered with rooting. The quantity of this basal callus was minimal when mTR was used. Increasing the concentration of either calcium or boron prevented the development of necrotic shoots. When the concentration of both elements was increased simultaneously, negative effects on both growth and STN were observed. Using 6 mM Ca in half-strength MS medium was optimum. Boron was toxic at higher (0.4 and 0.5 mM) concentrations. Plantlets rooted readily in half-strength cytokinin-free MS media supplemented with 2.5 mu M IAA. Rooted
Název v anglickém jazyce
Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium
Popis výsledku anglicky
Various experiments were conducted to characterize and control factors affecting shoot-tip necrosis (STN) in Harpagophytum procumbens. Higher cytokinin concentrations increased the incidence of STN and the problem was aggravated by the addition of auxin(IAA) to the multiplication medium. Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta-Topolin riboside (mTR). In the presence of auxin, plantlets produced basal callus that interfered with rooting. The quantity of this basal callus was minimal when mTR was used. Increasing the concentration of either calcium or boron prevented the development of necrotic shoots. When the concentration of both elements was increased simultaneously, negative effects on both growth and STN were observed. Using 6 mM Ca in half-strength MS medium was optimum. Boron was toxic at higher (0.4 and 0.5 mM) concentrations. Plantlets rooted readily in half-strength cytokinin-free MS media supplemented with 2.5 mu M IAA. Rooted
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EF - Botanika
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA522%2F06%2F0108" target="_blank" >GA522/06/0108: Nové cytokininové deriváty v rostlinách: isolace, identifikace a biologická aktivita</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2009
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
South African Journal of Botany
ISSN
0254-6299
e-ISSN
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Svazek periodika
75
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
6
Strana od-do
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Kód UT WoS článku
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EID výsledku v databázi Scopus
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