Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F10%3A10212711" target="_blank" >RIV/61989592:15310/10:10212711 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress

Popis výsledku v původním jazyce

Protoplast cultures are remarkable examples of plant cell dedifferentiation. The state of dedifferentiation is evidenced by changes in cell morphology, genome organization, as well as by the capability of protoplasts to differentiate into multiple typesof cells. The first change in the genome structure is connected with large-scale chromatin decondensation, affecting chromocenters involving various types of these repetitive sequences. This paper describes not only the de- and re-condensation of repetitive sequences, but it also compares the recondensation level of chromatin with the levels of oxidative stress which were decreased by using an antioxidant. We have demonstrated that the treatment of protoplasts with ascorbic acid not only decreased the level of oxidative stress but also positively stimulated the expression of the ascorbate peroxidase and catalase. It also led to a greater recondensation of the chromatin (when compared to the untreated protoplasts).

Název v anglickém jazyce

Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress

Popis výsledku anglicky

Protoplast cultures are remarkable examples of plant cell dedifferentiation. The state of dedifferentiation is evidenced by changes in cell morphology, genome organization, as well as by the capability of protoplasts to differentiate into multiple typesof cells. The first change in the genome structure is connected with large-scale chromatin decondensation, affecting chromocenters involving various types of these repetitive sequences. This paper describes not only the de- and re-condensation of repetitive sequences, but it also compares the recondensation level of chromatin with the levels of oxidative stress which were decreased by using an antioxidant. We have demonstrated that the treatment of protoplasts with ascorbic acid not only decreased the level of oxidative stress but also positively stimulated the expression of the ascorbate peroxidase and catalase. It also led to a greater recondensation of the chromatin (when compared to the untreated protoplasts).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/2E08018" target="_blank" >2E08018: Inovativní medializace zapojení žen do vědy a výzkumu jako faktor posílení znalostní společnosti a rovných příležitostí, a zvýšení konkurenceschopnosti v podmínkách rozvoje přírodovědných oborů.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimenal Botany

ISSN

0022-0957

e-ISSN

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Svazek periodika

61

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000279003600015

EID výsledku v databázi Scopus

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