Effect of exogenous hydrogen peroxide on biophoton emission from radish root cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F10%3A10217531" target="_blank" >RIV/61989592:15310/10:10217531 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of exogenous hydrogen peroxide on biophoton emission from radish root cells
Popis výsledku v původním jazyce
Biophotons spontaneously emitted from radish root cells were detected using highly sensitive photomultiplier tube. Freshly isolated radish root cells exhibited spontaneous photon emission of about 4 counts s_1. Addition of hydrogen peroxide to the cellscaused significant enhancement in biophoton emission to about 500 counts s_1. Removal of molecular oxygen using glucose/glucose oxidase systém and scavengering of reactive oxygen species by reducing agents such are sodium ascorbate and cysteine completely diminished biophoton emission. Spectral analysis of the hydrogen peroxide-induced biophoton emission indicates that biophotons are emitted mainly in greenered region of the spectra. The data provided by electron paramagnetic resonance spin-trapping technique showed that formation of singlet oxygen observed after addition of H2O2 correlates with enhancement in biophoton emission. These observations provide direct evidence that singlet oxygen is involved in biophoton emission from radish
Název v anglickém jazyce
Effect of exogenous hydrogen peroxide on biophoton emission from radish root cells
Popis výsledku anglicky
Biophotons spontaneously emitted from radish root cells were detected using highly sensitive photomultiplier tube. Freshly isolated radish root cells exhibited spontaneous photon emission of about 4 counts s_1. Addition of hydrogen peroxide to the cellscaused significant enhancement in biophoton emission to about 500 counts s_1. Removal of molecular oxygen using glucose/glucose oxidase systém and scavengering of reactive oxygen species by reducing agents such are sodium ascorbate and cysteine completely diminished biophoton emission. Spectral analysis of the hydrogen peroxide-induced biophoton emission indicates that biophotons are emitted mainly in greenered region of the spectra. The data provided by electron paramagnetic resonance spin-trapping technique showed that formation of singlet oxygen observed after addition of H2O2 correlates with enhancement in biophoton emission. These observations provide direct evidence that singlet oxygen is involved in biophoton emission from radish
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
BO - Biofyzika
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plant Physiology and Biochemistry
ISSN
0981-9428
e-ISSN
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Svazek periodika
2-3
Číslo periodika v rámci svazku
48
Stát vydavatele periodika
FR - Francouzská republika
Počet stran výsledku
7
Strana od-do
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Kód UT WoS článku
000276720900006
EID výsledku v databázi Scopus
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