Determination of rosiglitazone and metformin in human serum by CE-ESI-MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F11%3A33117310" target="_blank" >RIV/61989592:15310/11:33117310 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jssc.201000823" target="_blank" >http://dx.doi.org/10.1002/jssc.201000823</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201000823" target="_blank" >10.1002/jssc.201000823</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of rosiglitazone and metformin in human serum by CE-ESI-MS

Popis výsledku v původním jazyce

A new method for the determination of anti-diabetic drugs metformin and rosiglitazone based on the use of capillary electrophoresis with electrospray mass spectrometry was developed. The proposed method allowed their separation within 11 min by using 50mM formic acid at 120 kV. Positive electrospray ionization and selected ion monitoring [M1H]1 of metformin (m/z5130) and rosiglitazone (m/z5358) were performed. Several important experimental parameters influencing electrospray ionization of metformin androsiglitazone were studied. The final composition of sheath liquid was water/methanol/formic acid (50:49.5:0.5, v/v/v), at a flow rate of 2 mL/min. The developed method was applied for the determination of metformin and rosiglitazone simultaneously in human serum after protein precipitation with acetonitrile. The limits of detection of developed method were 4.42 and 2.14 ng/mL for rosiglitazone and for metformin, respectively, which is sufficient for therapeutic serum concentration leve

Název v anglickém jazyce

Determination of rosiglitazone and metformin in human serum by CE-ESI-MS

Popis výsledku anglicky

A new method for the determination of anti-diabetic drugs metformin and rosiglitazone based on the use of capillary electrophoresis with electrospray mass spectrometry was developed. The proposed method allowed their separation within 11 min by using 50mM formic acid at 120 kV. Positive electrospray ionization and selected ion monitoring [M1H]1 of metformin (m/z5130) and rosiglitazone (m/z5358) were performed. Several important experimental parameters influencing electrospray ionization of metformin androsiglitazone were studied. The final composition of sheath liquid was water/methanol/formic acid (50:49.5:0.5, v/v/v), at a flow rate of 2 mL/min. The developed method was applied for the determination of metformin and rosiglitazone simultaneously in human serum after protein precipitation with acetonitrile. The limits of detection of developed method were 4.42 and 2.14 ng/mL for rosiglitazone and for metformin, respectively, which is sufficient for therapeutic serum concentration leve

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F03.0058" target="_blank" >ED2.1.00/03.0058: Regionální centrum pokročilých technologií a materiálů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

1167-1173

Kód UT WoS článku

000290635400010

EID výsledku v databázi Scopus

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