Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana (Musa spp. AAA)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F11%3A33118111" target="_blank" >RIV/61989592:15310/11:33118111 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1471-2229-11-38" target="_blank" >http://dx.doi.org/10.1186/1471-2229-11-38</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1471-2229-11-38" target="_blank" >10.1186/1471-2229-11-38</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana (Musa spp. AAA)

Popis výsledku v původním jazyce

In this study, the localization and function of hydroxyproline rich glycoproteins in embryogenic cells (ECs) and somatic embryos of banana were investigated by using immunobloting and immunocytochemistry with monoclonal JIM11 and JIM20 antibodies as wellas by treatment with 3,4-dehydro-L-proline (3,4-DHP, an inhibitor of extensin biosynthesis), and by immunomodulation with the JIM11 antibody. Immunofluorescence labelling of JIM11 and JIM20 hydroxyproline rich glycoprotein epitopes was prominent mainlyin early embryogenic cells as well as in various developmental stages of somatic embryos showing strong labelling especially in their cell walls and outer surface layer, so-called extracellular matrix (ECM). This hydroxyproline rich glycoprotein signal at embryo surfaces decreased and/or fully disappeared during later developmental stages (e. g. pear-shaped and cotyledonary stages) of embryos. In these later developmental embryogenic stages, however, new prominent hydroxyproline rich gly

Název v anglickém jazyce

Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana (Musa spp. AAA)

Popis výsledku anglicky

In this study, the localization and function of hydroxyproline rich glycoproteins in embryogenic cells (ECs) and somatic embryos of banana were investigated by using immunobloting and immunocytochemistry with monoclonal JIM11 and JIM20 antibodies as wellas by treatment with 3,4-dehydro-L-proline (3,4-DHP, an inhibitor of extensin biosynthesis), and by immunomodulation with the JIM11 antibody. Immunofluorescence labelling of JIM11 and JIM20 hydroxyproline rich glycoprotein epitopes was prominent mainlyin early embryogenic cells as well as in various developmental stages of somatic embryos showing strong labelling especially in their cell walls and outer surface layer, so-called extracellular matrix (ECM). This hydroxyproline rich glycoprotein signal at embryo surfaces decreased and/or fully disappeared during later developmental stages (e. g. pear-shaped and cotyledonary stages) of embryos. In these later developmental embryogenic stages, however, new prominent hydroxyproline rich gly

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0007%2F01%2F01" target="_blank" >ED0007/01/01: Centrum regionu Haná pro biotechnologický a zemědělský výzkum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

B M C Plant Biology

ISSN

1471-2229

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

38

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

38-49

Kód UT WoS článku

000288038300001

EID výsledku v databázi Scopus

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