ER disruption and GFP degradation during non-regenerable transformation of flax with Agrobacterium tumefaciens

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33140446" target="_blank" >RIV/61989592:15310/12:33140446 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/12:00379091

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00709-010-0261-2" target="_blank" >http://dx.doi.org/10.1007/s00709-010-0261-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00709-010-0261-2" target="_blank" >10.1007/s00709-010-0261-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

ER disruption and GFP degradation during non-regenerable transformation of flax with Agrobacterium tumefaciens

Popis výsledku v původním jazyce

Flax is considered as plant species susceptible to Agrobacterium-mediated genetic transformation. In this study, stability of flax transformation by Agrobacterium rhizogenes versus Agrobacterium tumefaciens was tested by using combined selection for antibiotic resistance and visual selection of green fluorescent protein (GFP)-fusion reporter targeted to the endoplasmic reticulum (ER). Transformation with A. rhizogenes was stable for over 2 years, whereas transformation by A. tumefaciens resulted in non-regenerable stable transformation which was restricted solely to transgenic callus and lasted only 6-8 weeks. However, shoots regenerated from this callus appeared to be non-transgenic. Importantly, callus and root cells stably transformed with A. rhizogenes showed typical regular organization and dynamics of ER as visualized by GFP-ER marker. On the other hand, callus cells transformed with A. tumefaciens showed disintegrated ER structure and impaired dynamics which was accompanied with

Název v anglickém jazyce

ER disruption and GFP degradation during non-regenerable transformation of flax with Agrobacterium tumefaciens

Popis výsledku anglicky

Flax is considered as plant species susceptible to Agrobacterium-mediated genetic transformation. In this study, stability of flax transformation by Agrobacterium rhizogenes versus Agrobacterium tumefaciens was tested by using combined selection for antibiotic resistance and visual selection of green fluorescent protein (GFP)-fusion reporter targeted to the endoplasmic reticulum (ER). Transformation with A. rhizogenes was stable for over 2 years, whereas transformation by A. tumefaciens resulted in non-regenerable stable transformation which was restricted solely to transgenic callus and lasted only 6-8 weeks. However, shoots regenerated from this callus appeared to be non-transgenic. Importantly, callus and root cells stably transformed with A. rhizogenes showed typical regular organization and dynamics of ER as visualized by GFP-ER marker. On the other hand, callus cells transformed with A. tumefaciens showed disintegrated ER structure and impaired dynamics which was accompanied with

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0007%2F01%2F01" target="_blank" >ED0007/01/01: Centrum regionu Haná pro biotechnologický a zemědělský výzkum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Protoplasma: an international journal of cell biology

ISSN

0033-183X

e-ISSN

—

Svazek periodika

249

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

11

Strana od-do

53-63

Kód UT WoS článku

000298754000006

EID výsledku v databázi Scopus

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