Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33140482" target="_blank" >RIV/61989592:15310/12:33140482 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion

Popis výsledku v původním jazyce

This paper reports on haploid and mixoploid protoplasts in the genus Cucumis. The mixoploid plants were obtained after oryzalin treatments and the mesophyll protoplasts were isolated and cultuvated. The isolation and cultivation of the cucumber and muskmelon haploid protoplasts from young-stage pollen grains were improved. Somatic hybridization (fusion) between mixoploid cucumber protoplasts and muskmelon mesophyll and callus protoplasts by chemical fusion with polyethylene glycol (PEG, 6000) was performed for the first time. Heterofusants were observed and developed into microcolonies. Gametosomatic hybridization between mixoploid cucumber protoplasts and pollen muskmelon protoplasts was performed for the first time. heterofusants and first cell division were observed. Different ploidy, especially the mixoploid character of isolated protoplasts, has positive influence on protoplasts isolation and the following fusion.

Název v anglickém jazyce

Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion

Popis výsledku anglicky

This paper reports on haploid and mixoploid protoplasts in the genus Cucumis. The mixoploid plants were obtained after oryzalin treatments and the mesophyll protoplasts were isolated and cultuvated. The isolation and cultivation of the cucumber and muskmelon haploid protoplasts from young-stage pollen grains were improved. Somatic hybridization (fusion) between mixoploid cucumber protoplasts and muskmelon mesophyll and callus protoplasts by chemical fusion with polyethylene glycol (PEG, 6000) was performed for the first time. Heterofusants were observed and developed into microcolonies. Gametosomatic hybridization between mixoploid cucumber protoplasts and pollen muskmelon protoplasts was performed for the first time. heterofusants and first cell division were observed. Different ploidy, especially the mixoploid character of isolated protoplasts, has positive influence on protoplasts isolation and the following fusion.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Botany and Food Quality-Angewandte Botanik

ISSN

1613-9216

e-ISSN

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Svazek periodika

85

Číslo periodika v rámci svazku

85

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

64-72

Kód UT WoS článku

000310189700009

EID výsledku v databázi Scopus

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