Biotechnological methods for in vitro propagation in willows (Salix spp.)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33140798" target="_blank" >RIV/61989592:15310/12:33140798 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.2478/s11535-012-0069-5" target="_blank" >http://dx.doi.org/10.2478/s11535-012-0069-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/s11535-012-0069-5" target="_blank" >10.2478/s11535-012-0069-5</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Biotechnological methods for in vitro propagation in willows (Salix spp.)
Popis výsledku v původním jazyce
Many populations of high-mountainous relic dioecious willows in Central Europe only consist of female individuals and are thus limited in their reproductive potential. We completed micropropagation experiments with shoot apexes and nodal segments of common and endangered willow (Salix) species, which can help to reintroduce autochthonous genotypes to their natural sites. Until recently, cultivation of green young shoot apexes of S. alba and S. lapponum showed the highest percentage of regeneration. We successfully applied the two-times-sterilisation due to high contamination of natural explants. The OK medium was the most efficient culture medium. In vitro propagation of willows with unisexual catkins, anther and ovule cultures were tested and optimised. Isolated anthers were cultivated on selected media and then microcallus and calluses of S. caprea and calluses of S. viminalis were formed on the A medium. Among various tested and optimised media for the ovule culture, the CP medium w
Název v anglickém jazyce
Biotechnological methods for in vitro propagation in willows (Salix spp.)
Popis výsledku anglicky
Many populations of high-mountainous relic dioecious willows in Central Europe only consist of female individuals and are thus limited in their reproductive potential. We completed micropropagation experiments with shoot apexes and nodal segments of common and endangered willow (Salix) species, which can help to reintroduce autochthonous genotypes to their natural sites. Until recently, cultivation of green young shoot apexes of S. alba and S. lapponum showed the highest percentage of regeneration. We successfully applied the two-times-sterilisation due to high contamination of natural explants. The OK medium was the most efficient culture medium. In vitro propagation of willows with unisexual catkins, anther and ovule cultures were tested and optimised. Isolated anthers were cultivated on selected media and then microcallus and calluses of S. caprea and calluses of S. viminalis were formed on the A medium. Among various tested and optimised media for the ovule culture, the CP medium w
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EI - Biotechnologie a bionika
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Central European Journal of Biology
ISSN
1895-104X
e-ISSN
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Svazek periodika
7
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
10
Strana od-do
931-940
Kód UT WoS článku
000307287100018
EID výsledku v databázi Scopus
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