Plant Aurora kinases play a role in maintenance of primary meristems and control of endoreduplication
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33140799" target="_blank" >RIV/61989592:15310/12:33140799 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61388971:_____/12:00380510 RIV/61389030:_____/12:00380510
Výsledek na webu
<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2011.03989.x/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2011.03989.x/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1469-8137.2011.03989.x" target="_blank" >10.1111/j.1469-8137.2011.03989.x</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Plant Aurora kinases play a role in maintenance of primary meristems and control of endoreduplication
Popis výsledku v původním jazyce
The conserved family of Aurora kinases has multiple functions during mitosis. The roles of plant Aurora kinases have been characterized using inhibitor treatments. We down-regulated Aurora kinases in Arabidopsis thaliana using RNA interference (RNAi). Wecarried out a detailed phenotypic analysis of Aurora RNAi plants, biochemical and microscopic studies of AtAurora1 kinase together with AtTPX2 (targeting protein for Xklp2) and ?-tubulin. Cell division defects were observed in plants with reduced expression of Aurora kinases. Furthermore, the maintenance of primary meristems was compromised and RNAi seedlings entered endoreduplication prematurely. AtAurora1, its activator AtTPX2, and ?-tubulin were associated with microtubules in vitro; they were attached to regrowing kinetochore microtubules and colocalized on spindle microtubules and with a subset of early phragmoplast microtubules. Only the AtAurora1 kinase was translocated to the area of the cell plate. RNAi silencing of Aurora kin
Název v anglickém jazyce
Plant Aurora kinases play a role in maintenance of primary meristems and control of endoreduplication
Popis výsledku anglicky
The conserved family of Aurora kinases has multiple functions during mitosis. The roles of plant Aurora kinases have been characterized using inhibitor treatments. We down-regulated Aurora kinases in Arabidopsis thaliana using RNA interference (RNAi). Wecarried out a detailed phenotypic analysis of Aurora RNAi plants, biochemical and microscopic studies of AtAurora1 kinase together with AtTPX2 (targeting protein for Xklp2) and ?-tubulin. Cell division defects were observed in plants with reduced expression of Aurora kinases. Furthermore, the maintenance of primary meristems was compromised and RNAi seedlings entered endoreduplication prematurely. AtAurora1, its activator AtTPX2, and ?-tubulin were associated with microtubules in vitro; they were attached to regrowing kinetochore microtubules and colocalized on spindle microtubules and with a subset of early phragmoplast microtubules. Only the AtAurora1 kinase was translocated to the area of the cell plate. RNAi silencing of Aurora kin
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
New Phytologist (print)
ISSN
0028-646X
e-ISSN
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Svazek periodika
193
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
15
Strana od-do
590-604
Kód UT WoS článku
000298984900006
EID výsledku v databázi Scopus
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