Avidin Functionalized Maghernite Nanoparticles and Their Application for Recombinant Human Biotinyl-SERCA Purification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33141654" target="_blank" >RIV/61989592:15310/12:33141654 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1021/la303148u" target="_blank" >http://dx.doi.org/10.1021/la303148u</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/la303148u" target="_blank" >10.1021/la303148u</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Avidin Functionalized Maghernite Nanoparticles and Their Application for Recombinant Human Biotinyl-SERCA Purification

Popis výsledku v původním jazyce

We report on the surface characterization, functionalization, and application of stable water suspensions of novel surface active maghemite narioparticles (SAMNs), characterized by a diameter of 11 +/- 2 nm and possessing peculiar colloidal properties and surface interactions. These features permitted the acquisition of titration curves and aqueous UV-vis spectra and suggested a role played by surface under-coordinated iron atoms. This new class of nanoparticles was obtained through an easy, inexpensive, one-step, green procedure and functionalized with ligands of high biotechnological interest, such as biotin and avidin, by simple incubation in aqueous solution. Bound avidin was determined by measuring the disappearance of free avidin absorbance at 280 nm, as a function of increasing nanoparticle concentration, showing the presence of 10 +/- 3 avidin molecules per nanoparticle. The biological activity of the SAMN@avidin complex was evaluated and the number of available biotin binding

Název v anglickém jazyce

Avidin Functionalized Maghernite Nanoparticles and Their Application for Recombinant Human Biotinyl-SERCA Purification

Popis výsledku anglicky

We report on the surface characterization, functionalization, and application of stable water suspensions of novel surface active maghemite narioparticles (SAMNs), characterized by a diameter of 11 +/- 2 nm and possessing peculiar colloidal properties and surface interactions. These features permitted the acquisition of titration curves and aqueous UV-vis spectra and suggested a role played by surface under-coordinated iron atoms. This new class of nanoparticles was obtained through an easy, inexpensive, one-step, green procedure and functionalized with ligands of high biotechnological interest, such as biotin and avidin, by simple incubation in aqueous solution. Bound avidin was determined by measuring the disappearance of free avidin absorbance at 280 nm, as a function of increasing nanoparticle concentration, showing the presence of 10 +/- 3 avidin molecules per nanoparticle. The biological activity of the SAMN@avidin complex was evaluated and the number of available biotin binding

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Langmuir: the A C S journal of surfaces and colloids

ISSN

0743-7463

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

43

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

15392-15401

Kód UT WoS článku

000310484300032

EID výsledku v databázi Scopus

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