Liquid chromatography-tandem mass spectrometry method for determination of five antidepressants and four atypical antipsychotics and their main metabolites in human serum

Kód výsledku v IS VaVaI

RIV/61989592:15310/12:33142285 - isvavai.cz

Nalezeny alternativní kódy

RIV/00843989:_____/12:00102955

Výsledek na webu

http://dx.doi.org/10.1016/j.jchromb.2012.09.009

DOI - Digital Object Identifier

10.1016/j.jchromb.2012.09.009

Jazyk výsledku

angličtina

Název v původním jazyce

Liquid chromatography-tandem mass spectrometry method for determination of five antidepressants and four atypical antipsychotics and their main metabolites in human serum

Popis výsledku v původním jazyce

The rapid and simple ultra performance liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination parent drugs: sertraline, fluoxetine, citalopram, paroxetine, venlafaxine, clozapine, olanzapine, quetiapine, risperidone, and their active and nonacrive metabolites N-desmethylsertraline, norfluoxetine, desmethylcitalopram, didemethylcitalopram. N-desmethylvenlafaxine, O-desmethylvenlafaxine, N-desmethylclozapine, N-desmethylolanzapine, 2-hydroxyolanzapine and 9-hydroxyrisperidone in human serum. Precipitation of serum proteins was performed with a precipitation reagent consisting of 0.05% solution of ZnSO4 center dot 7H(2)O in acetonitrile/methanol (40:60, v/v). Alprenolol was used as an internal standard. Chromatographic separation was carried out on a BEH C18 column using gradient elution mobile phase A (2 mmol/L ammonium acetate, 0.1% formic acid in 5% acetonitrile, v/v/v) and B (2 mmol/L ammonium acetate, 0.1% formic acid in 95% a

Název v anglickém jazyce

Liquid chromatography-tandem mass spectrometry method for determination of five antidepressants and four atypical antipsychotics and their main metabolites in human serum

Popis výsledku anglicky

The rapid and simple ultra performance liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination parent drugs: sertraline, fluoxetine, citalopram, paroxetine, venlafaxine, clozapine, olanzapine, quetiapine, risperidone, and their active and nonacrive metabolites N-desmethylsertraline, norfluoxetine, desmethylcitalopram, didemethylcitalopram. N-desmethylvenlafaxine, O-desmethylvenlafaxine, N-desmethylclozapine, N-desmethylolanzapine, 2-hydroxyolanzapine and 9-hydroxyrisperidone in human serum. Precipitation of serum proteins was performed with a precipitation reagent consisting of 0.05% solution of ZnSO4 center dot 7H(2)O in acetonitrile/methanol (40:60, v/v). Alprenolol was used as an internal standard. Chromatographic separation was carried out on a BEH C18 column using gradient elution mobile phase A (2 mmol/L ammonium acetate, 0.1% formic acid in 5% acetonitrile, v/v/v) and B (2 mmol/L ammonium acetate, 0.1% formic acid in 95% a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

ED2.1.00/03.0058: Regionální centrum pokročilých technologií a materiálů

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

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Svazek periodika

907

Číslo periodika v rámci svazku

říjen

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

101-107

Kód UT WoS článku

000310256500015

EID výsledku v databázi Scopus

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