Small CAB-like proteins prevent formation of singlet oxygen in the damaged Photosystem II complex of the cyanobacterium Synechocystis sp. PCC 6803

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142608" target="_blank" >RIV/61989592:15310/12:33142608 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/12:00389730 RIV/60076658:12310/12:43883661

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1365-3040.2011.02454.x" target="_blank" >http://dx.doi.org/10.1111/j.1365-3040.2011.02454.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1365-3040.2011.02454.x" target="_blank" >10.1111/j.1365-3040.2011.02454.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Small CAB-like proteins prevent formation of singlet oxygen in the damaged Photosystem II complex of the cyanobacterium Synechocystis sp. PCC 6803

Popis výsledku v původním jazyce

The cyanobacterial small CAB-like proteins (SCPs) are a single helix membrane proteins mostly associated with the Photosystem II (PSII) complex that accumulate under stress conditions. Their function is still ambiguous although they are assumed to regulate chlorophyll (Chl) biosynthesis and/or to protect PSII against oxidative damage. In this study, the effect of SCPs on the PSII-specific light-induced damage and generation of singlet oxygen (1O2) was assessed in the strains of the cyanobacterium Synechocystis sp. PCC 6803 lacking Photosystem I (PSI-less strain) or lacking PSI together with all SCPs (PSI-less/scpABCDE? strain). The light-induced oxidative modifications of the PSII D1 protein reflected by a mobility shift of the D1 protein and by generation of a D1-cytochrome b-559 adduct were more pronounced in the PSI-less/scpABCDE? strain. This increased protein oxidation correlated with a faster formation of 1O2 as detected by the green fluorescence of Singlet Oxygen Sensor Green as

Název v anglickém jazyce

Small CAB-like proteins prevent formation of singlet oxygen in the damaged Photosystem II complex of the cyanobacterium Synechocystis sp. PCC 6803

Popis výsledku anglicky

The cyanobacterial small CAB-like proteins (SCPs) are a single helix membrane proteins mostly associated with the Photosystem II (PSII) complex that accumulate under stress conditions. Their function is still ambiguous although they are assumed to regulate chlorophyll (Chl) biosynthesis and/or to protect PSII against oxidative damage. In this study, the effect of SCPs on the PSII-specific light-induced damage and generation of singlet oxygen (1O2) was assessed in the strains of the cyanobacterium Synechocystis sp. PCC 6803 lacking Photosystem I (PSI-less strain) or lacking PSI together with all SCPs (PSI-less/scpABCDE? strain). The light-induced oxidative modifications of the PSII D1 protein reflected by a mobility shift of the D1 protein and by generation of a D1-cytochrome b-559 adduct were more pronounced in the PSI-less/scpABCDE? strain. This increased protein oxidation correlated with a faster formation of 1O2 as detected by the green fluorescence of Singlet Oxygen Sensor Green as

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant, Cell and Environment

ISSN

0140-7791

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

.

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

"806?818"

Kód UT WoS článku

000301055100011

EID výsledku v databázi Scopus

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