LC/MS metabolic study on quercetin and taxifolin galloyl esters using human hepatocytes as toxicity and biotransformation in vitro cell model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33144017" target="_blank" >RIV/61989592:15310/13:33144017 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/13:00424179 RIV/61989592:15110/13:33144017

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jpba.2013.07.045" target="_blank" >http://dx.doi.org/10.1016/j.jpba.2013.07.045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jpba.2013.07.045" target="_blank" >10.1016/j.jpba.2013.07.045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

LC/MS metabolic study on quercetin and taxifolin galloyl esters using human hepatocytes as toxicity and biotransformation in vitro cell model

Popis výsledku v původním jazyce

Galloyl esters of quercetin and taxifolin have been recently prepared semisynthetically as part of work towards modifying the solubility and modulating the biological aktivity of these natural flavonoids. In this paper we focused on the liquid chromatography?mass spektrometry (LC?MS) profiling of metabolites of 3-O-galloylquercetin and 7-O-galloyltaxifolin using human hepatocytes as the in vitro cell model. A subtoxic concentration (50 M) was used for both compounds and the formation of metabolites wasmonitored for 2 h in hepatocytes and cultivation medium separately. Using negative electrospray ionization-quadrupole time-of-flight mass spektrometry (ESI-QqTOF MS), we identified different biotransformation patterns for the studied compounds. 3-O-Galloylquercetin is metabolized directly to glucuronides and methyl derivatives. In contrast, 7-O-galloyltaxifolin is oxidized to 7-O-galloylquercetin or cleaved to taxifolin, and consequently the products formed are sulfated or glucuronidated

Název v anglickém jazyce

LC/MS metabolic study on quercetin and taxifolin galloyl esters using human hepatocytes as toxicity and biotransformation in vitro cell model

Popis výsledku anglicky

Galloyl esters of quercetin and taxifolin have been recently prepared semisynthetically as part of work towards modifying the solubility and modulating the biological aktivity of these natural flavonoids. In this paper we focused on the liquid chromatography?mass spektrometry (LC?MS) profiling of metabolites of 3-O-galloylquercetin and 7-O-galloyltaxifolin using human hepatocytes as the in vitro cell model. A subtoxic concentration (50 M) was used for both compounds and the formation of metabolites wasmonitored for 2 h in hepatocytes and cultivation medium separately. Using negative electrospray ionization-quadrupole time-of-flight mass spektrometry (ESI-QqTOF MS), we identified different biotransformation patterns for the studied compounds. 3-O-Galloylquercetin is metabolized directly to glucuronides and methyl derivatives. In contrast, 7-O-galloyltaxifolin is oxidized to 7-O-galloylquercetin or cleaved to taxifolin, and consequently the products formed are sulfated or glucuronidated

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Pharmaceutical and Biomedical Analysis

ISSN

0731-7085

e-ISSN

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Svazek periodika

86

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

135-142

Kód UT WoS článku

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EID výsledku v databázi Scopus

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