Vesicular Trafficking and Stress Response Coupled to PI3K Inhibition by LY294002 as Revealed by Proteomic and Cell Biological Analysis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33146711" target="_blank" >RIV/61989592:15310/13:33146711 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1021/pr400466x" target="_blank" >http://dx.doi.org/10.1021/pr400466x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/pr400466x" target="_blank" >10.1021/pr400466x</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Vesicular Trafficking and Stress Response Coupled to PI3K Inhibition by LY294002 as Revealed by Proteomic and Cell Biological Analysis
Popis výsledku v původním jazyce
LY294002 is a synthetic quercetin-like compound, which, unlike wortmannin, is more specific inhibitor of phosphatidylinositol 3-kinase (PI3K). It inhibits endocytosis and vacuolar transport. We report here on the proteome-wide effects of LY294002 on Arabidopsis roots focusing on proteins involved in vesicular trafficking and stress response. At the subcellular level, LY294002 caused swelling and clustering of late endosomes leading to inhibition of vacuolar transport. At the proteome level, this compound caused changes in abundances of proteins categorized to 10 functional classes. Among proteins involved in vesicular trafficking, a small GTPase ARFA1f was more abundant, indicating its possible contribution to the aggregation and fusion of late endosomes triggered by LY294002. Our study provides new information on storage proteins and vacuolar hydrolases in vegetative tissues treated by LY294002. Vacuolar hydrolases were downregulated, while storage proteins were more abundant, suggest
Název v anglickém jazyce
Vesicular Trafficking and Stress Response Coupled to PI3K Inhibition by LY294002 as Revealed by Proteomic and Cell Biological Analysis
Popis výsledku anglicky
LY294002 is a synthetic quercetin-like compound, which, unlike wortmannin, is more specific inhibitor of phosphatidylinositol 3-kinase (PI3K). It inhibits endocytosis and vacuolar transport. We report here on the proteome-wide effects of LY294002 on Arabidopsis roots focusing on proteins involved in vesicular trafficking and stress response. At the subcellular level, LY294002 caused swelling and clustering of late endosomes leading to inhibition of vacuolar transport. At the proteome level, this compound caused changes in abundances of proteins categorized to 10 functional classes. Among proteins involved in vesicular trafficking, a small GTPase ARFA1f was more abundant, indicating its possible contribution to the aggregation and fusion of late endosomes triggered by LY294002. Our study provides new information on storage proteins and vacuolar hydrolases in vegetative tissues treated by LY294002. Vacuolar hydrolases were downregulated, while storage proteins were more abundant, suggest
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Proteome Research
ISSN
1535-3893
e-ISSN
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Svazek periodika
12
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
14
Strana od-do
4435-4448
Kód UT WoS článku
000326320300015
EID výsledku v databázi Scopus
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