A systematic comparison of embryogenic and non-embryogenic cells of banana (Musa spp. AAA): Ultrastructural, biochemical and cell wall component analyses
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33146844" target="_blank" >RIV/61989592:15310/13:33146844 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S0304423813002355" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0304423813002355</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.scienta.2013.05.008" target="_blank" >10.1016/j.scienta.2013.05.008</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A systematic comparison of embryogenic and non-embryogenic cells of banana (Musa spp. AAA): Ultrastructural, biochemical and cell wall component analyses
Popis výsledku v původním jazyce
Plant regeneration via somatic embryogenesis is essential for improvement of vegetatively propagated banana (Musa spp. AAA) through biotechnological techniques. A systematic comparison of embryogenic cells (ECs) and non-embryogenic cells (NECs) of bananawas performed in the present study. Immuno-dot blotting, immunoflourescence labeling, biochemical and ultrastructural analyses were employed to study differences between ECs and NECs. Both immuno-dot analysis and immunofluorescence labeling revealed a higher abundance of fucosylated xyloglucans in cell walls of ECs. Arabinogalactan-protein (AGP) epitopes recognized by LM2 and NH4(center dot)3E5 antibodies were more abundant in NECs. On the other hand, AGPs recognized by JIM16, MAC204 and MAC207 antibodies were more abundant in ECs. Biochemical analyses revealed that the content of total soluble sugars and reducing sugars, as well as levels of indole-3-acetic acid, abscisic acid and zeatin riboside were significantly higher in ECs in co
Název v anglickém jazyce
A systematic comparison of embryogenic and non-embryogenic cells of banana (Musa spp. AAA): Ultrastructural, biochemical and cell wall component analyses
Popis výsledku anglicky
Plant regeneration via somatic embryogenesis is essential for improvement of vegetatively propagated banana (Musa spp. AAA) through biotechnological techniques. A systematic comparison of embryogenic cells (ECs) and non-embryogenic cells (NECs) of bananawas performed in the present study. Immuno-dot blotting, immunoflourescence labeling, biochemical and ultrastructural analyses were employed to study differences between ECs and NECs. Both immuno-dot analysis and immunofluorescence labeling revealed a higher abundance of fucosylated xyloglucans in cell walls of ECs. Arabinogalactan-protein (AGP) epitopes recognized by LM2 and NH4(center dot)3E5 antibodies were more abundant in NECs. On the other hand, AGPs recognized by JIM16, MAC204 and MAC207 antibodies were more abundant in ECs. Biochemical analyses revealed that the content of total soluble sugars and reducing sugars, as well as levels of indole-3-acetic acid, abscisic acid and zeatin riboside were significantly higher in ECs in co
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED0007%2F01%2F01" target="_blank" >ED0007/01/01: Centrum regionu Haná pro biotechnologický a zemědělský výzkum</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Sciencia Horticulturae
ISSN
0304-4238
e-ISSN
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Svazek periodika
159
Číslo periodika v rámci svazku
30.7.2013
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
8
Strana od-do
178-185
Kód UT WoS článku
000322932400025
EID výsledku v databázi Scopus
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